10: Molecular Luminescence Spectrometry
- Page ID
- 220474
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Luminescence is emission of light by a substance not resulting from heat; it is thus a form of cold-body radiation. It can be caused by chemical reactions, electrical energy, subatomic motions, or stress on a crystal, which all are ultimately caused by Spontaneous emission. This distinguishes luminescence from incandescence, which is light emitted by a substance as a result of heating.
- 10.1: Fluorescence and Phosphorescence
- Fluorescence and phosphorescence are types of molecular luminescence methods. A molecule of analyte absorbs a photon and excites a species. The emission spectrum can provide qualitative and quantitative analysis. The term fluorescence and phosphorescence are usually referred as photoluminescence because both are alike in excitation brought by absorption of a photon.
- 10.2: Fluorescence and Phosphorescence Instrumentation
- Meant to be like Skoog's Instrumental Analysis chapter 15 section B
- 10.3: Applications of Photoluminescence Methods
- This section is to be like section 15c in skoog but expanded to include a discussion of the Stokes shift and the detection advantage, quenching, polariazation analysis, and fluorescence microscopy.
Thumbanil: Chemiluminescence after a reaction of hydrogen peroxide and luminol. This is an image from video https://youtu.be/8_82cNtZSQE. Image used with permission (CC BY-SA 4.0; Tavo Romann).