Biochem Meeting Minutes from Spring Semester 2017
- Page ID
- 77875
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)1. Date, Time : Friday 12:30 - 2 PM, 2/10/17
Location: DVC Horticulture
Attendees: Ryan and Dang
Topics of Discussion: Gave Dang a tour of the greenhouse and horticulture department.
2. Date, Time : Friday 12:30 - 2 PM, 2/17/17
Location: DVC Horticulture
3. Date, Time : Friday 12:30 - 2 PM, 2/24/17
Location: DVC Horticulture
Attendees: Ryan, Dang, Peter, Keren and Ivan
Topics of Discussion: Plants selections and measurements
- Discussed different species of lettuce and where to purchase it (likely Baker Creek).
- Peter will do some research, provide with recommendation for aeroponic species, and will be in charge of ordering fresh seeds, once selected.
- Took Inventory of Horticulture Classroom and Greenhouse.
- Took measurements of green house area designated for project, dimensions of tank as well as internal and external enclosure.
4. Date, Time : Friday 12:30- 2:05 PM 3/3/17
Location: DVC Horticulture
Attendees: Ryan, Keren, Chris, Melvinna, Ivan, Peter, Dang
Topics of Discussion: Temperature, Protocol and Method Draft
- Peter will be looking into getting temperature recordings from Green House.
- Discussed design protocol and designed architecture for chloramine testing.
- Six different methods for chloramine breakdown:
A) Control (water + NH2Cl)
B) Ascorbic Acid or Sodium Asorbate
C) Aerated Water
D) Bacteria substrate
E) Bacteria substrate w/ aeration
F) REDOX/OZONE Pump
5. Date, Time : Friday 12:30-2:20 PM, 3/10/17
Location: DVC Horticulture
Attendees: Ryan, Keren, Chris, Peter, Dang
Topics of Discussion: Water Report, In-depth methods discussion
- Ryan presented Contra Costa Water Report and discussed the levels of chloramine and trihalomethanes in water. Further discussed the industry standard methods for finding chloramine and halos levels. -> Amperometric Titration, DPD Ferrous Titrimetric and DPD Colorimetric (Cost Prohibitive)
- Contra Costa Water states that they produce chloramine by combining chlorine and ammonia.
- pH range of 8.2-8.4 to make monochloramine. (Note pKa Cl- = -8)
- Discussed the types of by products R-NHCl from reaction and reactions with organic material (trihalomethane=CH4 with three H changed….chloroform…etc)
- Design of methods and protocols:
A) Production of chloramine will be as similar to water department as possible (ammonia + chlorine). We will generate similar by products as the water district(halos…) if we keep the equilibrium reaction in a pH of 8.2-8.4.
B) Economic Alternatives for chloramine indicators;
We will test Total Chlorine and Free Chlorine by using either
*Test Strips, Titration, Colorimeter
6. Date, Time : Friday 12:30-2:50 PM, 3/17/17
Location: DVC Horticulture
Attendees: Ryan, Keren, Chris, Peter, Dang
Topics of Discussion: Cleaning and Proof of Concept for Pump
- Began with cleaning up of fish tank and its surrounding areas of greenhouse.
- Removed spider webs and grime from ceiling and walls.
- Tank was scrubbed by brush and sponge several times in a solution of: H2O2 (30%), organic Kirkland dish soap and H2O.
- Tank was rinsed and vacuumed until all parts had clean water.
- Lightly wiped tank with paper towel.
- Left uncovered for tank to dry out.
- Peter will cover tank with garbage bags Monday.
- Built and tested airlift water pump proof of concept.
- Results: The straight pipe used functioned with marginal flow and water exchange likely due to the lack of a siphon (vacuum) action that is present in the complete design.
7. Date, Time : Friday 12:30-2:00 PM, 3/24/17
Location: DVC Horticulture
Attendees: Ryan, Keren, Melvinna, Ivan, Philippe, Dang
Topic of Discussion: Pump, finalize method of generating chloramine, finalize the methods to break chloramine, possible chloramine detectors
- Horticulture's pump cannot be used (the problem will be discussed on Wednesday)
- Possible Solutions: DC solar (Philippe), use the horticulture pump at night (Ryan)
- Generating Chloramine Water
NH3 + Cl2 --> NH2Cl (pH: 8.4)
1 : 4 (similar ratio to tap water)
add chloramine into DI water (total: 6 barrels)
- Methods for the breaking of chloramines
a. Ascorbic acid
b. Bacteria
c. Air
d. Air +bacteria
e. Ozone
>control: standing water (O2 from the surface will eventually break it down in a course of one week)
- Detecting chloramine concentration
a. Chlorine test strips (cheap, but not precise)- range of detection: 0 - 10 ppm
b. Ampoterametric (very expensive) - will not be discussed further
c. DPD chemical indicator- range of detection: 0- 3.4 ppm
> tap water's concentration is around 1.5 ppm
- Suggestions to maximize our resources:
Melvinna: Test all the results from the breaking of chloramine methods with the chlorine test strips first, since they are cheaper and can be easily obtained. The methods that shows little to no signs of breaking the chloramine down can be eliminated right away. So that the DPD test would only be used for the methods that are already proven to be efficient enough. Since it is expensive, the DPD will only be used as the determining factor of which method will be used in the future.
Ryan: Test all the results from the methods with both tests. This is because the strips are not accurate enough compare to the DPD method.
- Budget and Inventory
| Have | Possible Needs |
| 55 gallon drums | 15 M NH3 (Chemistry Stock Room) |
| ozone pump | OCl-/Cl2 gas (*will be discussed further) |
| 5 gallons air compressor | chlorine test strips ($15) |
| DPD test kit for titration ($65) | |
| DPD colorimetric or DPD test kit (hach.com- DPD and ampul) | |
| DI water | |
| ampule or cuvette kit | |
| pipette | |
| potassium permanganate standardization solution |
8. Date, Time : Friday 12:30-2:00 PM, 4/1/17
Location: DVC Horticulture
Attendees: Ryan, Keren, Melvinna, Ivan, Peter, Dang
Topics of Discussion: Run down of methods and inventory
- Fiji rock or live rock need 6 weeks to culture, might be for next semester
| Need to Order: | |
| KI or selenium arsenate --> standardizing | H2SO4 (adjust pH) |
| HOCl (monochlorine) | Ferrous Ammonium Std. (FAS) |
| NH3 (aq) (monochlorine) | Potassium Permanganate (Standard) |
| DPD indicator | Nano water for in cuvette |
| cuvettes | thioacetamide |
1. Generate the chloramine water
2. Standardized the solution with KI then place in cuvettes
3. Place in sample that have been undergo the 6 methods.
4. Add thioacetamide (to bind with other source of N)
5. Adjust the pH using H2SO4.
6. Add the DPD indicator.
7 Perform a DPD test with spectrophotometer (Chemistry Lab)
8. Perform another test with chlorine strips.
*All the finalized discussion in the minutes is posted on the designated area with more details (ex. methods/protocols)*