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13: Introduction to Ultraviolet/Visible Absorption Spectrometry

13: Introduction to Ultraviolet/Visible Absorption Spectrometry

Last updated

Aug 28, 2022
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- 12.4: Other X-Ray Methods
- 13.1: Transmittance and Absorbance

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13.1: Transmittance and Absorbance
As light passes through a sample, its power decreases as some of it is absorbed. This attenuation of radiation is described quantitatively by two separate, but related terms: transmittance and absorbance.
13.2: Beer's Law
Beer's law connects absorbance to the concentration of the absorbing species. In this section we derive Beer's law and consider some of its limitations.
13.3: Effect of Noise on Transmittance and Absorbance Measurements
In absorption spectroscopy, precision is limited by indeterminate errors—primarily instrumental noise—which are introduced when we measure absorbance. Precision generally is worse for low absorbances and for high absorbances . We might expect, therefore, that precision will vary with transmittance.
13.4: Instrumentation
As covered in Chapter 7, the basic instrumentation for absorbance measurements consists of a source of radiation, a means for selecting the wavelengths to use, a means for detecting the amount of light absorbed by the sample, and a means for processing and displaying the data. In this section we consider two other essential components of an instrument for measuring the absorbance of UV/Vis radiation by molecules: the optical path that connects the source to the detector and a means for placing t

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