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- https://chem.libretexts.org/Courses/BethuneCookman_University/BCU%3A_CH-346_Instrumental_Analysis/Chromatography/V._Chromatography/C._High_Performance_Liquid_Chromatography_(HPLC)High performance liquid chromatography is a powerful tool in analysis. This page looks at how it is carried out and shows how it uses the same principles as in thin layer chromatography and column chr...High performance liquid chromatography is a powerful tool in analysis. This page looks at how it is carried out and shows how it uses the same principles as in thin layer chromatography and column chromatography.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.08%3A_ProblemsThis page presents a comprehensive set of problems in chromatography and capillary zone electrophoresis (CZE). Key topics include calculating theoretical plates, resolving power, chromatographic resol...This page presents a comprehensive set of problems in chromatography and capillary zone electrophoresis (CZE). Key topics include calculating theoretical plates, resolving power, chromatographic resolution, retention indices, ion balances, and evaluating chromatographic data for various compounds. Problems engage chemical principles such as pH impact, calibration curves, and the Van Deemter equation.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Physical_Methods_in_Chemistry_and_Nano_Science_(Barron)/03%3A_Principles_of_Gas_Chromatography/3.02%3A_High_Performance_Liquid_chromatographyHigh-performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate the components in a mixture, and to identify and quantify each component. It was initially discove...High-performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate the components in a mixture, and to identify and quantify each component. It was initially discovered as an analytical technique in the early twentieth century and was first used to separate colored compounds. The word chromatography means color writing.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/28%3A_High-Performance_Liquid_ChromatographyIn high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column t...In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase. By adjusting conditions it usually is possible to design a separation so that analytes elute by themselves, even when the mixture is complex.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.05%3A_High-Performance_Liquid_ChromatographyThis page discusses High-Performance Liquid Chromatography (HPLC), a method for separating compounds in a liquid solution. HPLC involves key components: reservoirs for the mobile phase, a pump, an inj...This page discusses High-Performance Liquid Chromatography (HPLC), a method for separating compounds in a liquid solution. HPLC involves key components: reservoirs for the mobile phase, a pump, an injector, columns (analytical and guard), and detectors. It explains different separation techniques (liquid-solid adsorption, liquid-liquid partitioning, etc.), types of columns (packed and capillary), stationary and mobile phases, and their interactions.
- https://chem.libretexts.org/Under_Construction/Purgatory/Principles_of_Instrumental_Analysis_(Skoog_et_al.)_-_Under_Construction/28%3A_High-Performance_Liquid_ChromatographyHigh performance liquid chromatography has proven itself to very useful in many scientific fields, yet forces scientists to consistently choose between speed and resolution. Ultra High Performance Liq...High performance liquid chromatography has proven itself to very useful in many scientific fields, yet forces scientists to consistently choose between speed and resolution. Ultra High Performance Liquid Chromatography (UHPLC) eliminates the need to choose and creates a highly efficient method that is primarily based on small particle separations.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/28%3A_High-Performance_Liquid_Chromatography/28.03%3A_Instruments_for_Liquid_ChromatographyIn high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column t...In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase. In this section we consider each of these.
- https://chem.libretexts.org/Courses/British_Columbia_Institute_of_Technology/Chem_2305%3A_Biochemistry_Instrumental_Analysis/02%3A_Chromatography/2.03%3A_Liquid_Chromatography/2.3.01%3A_High-Performance_Liquid_ChromatographyHigh performance liquid chromatography has proven itself to very useful in many scientific fields, yet forces scientists to consistently choose between speed and resolution. Ultra High Performance Liq...High performance liquid chromatography has proven itself to very useful in many scientific fields, yet forces scientists to consistently choose between speed and resolution. Ultra High Performance Liquid Chromatography (UHPLC) eliminates the need to choose and creates a highly efficient method that is primarily based on small particle separations.
- https://chem.libretexts.org/Ancillary_Materials/Worksheets/Worksheets%3A_Analytical_Chemistry_II/HPLC_Case_Study%3A_Forensics_with_Chromatographic_Data_SetContributors and Attributions Amber Hupp (ahupp@holycross.edu) and Rebecca Davies, College of the Holy Cross Sourced from the Analytical Sciences Digital Library
- https://chem.libretexts.org/Ancillary_Materials/Worksheets/Worksheets%3A_Analytical_Chemistry_II/Literature_Article_Analysis%3A_HPLC_vs._GC-MSToward the end of the introduction, often in the last paragraph, it is common to transition from background information to a brief summary of the specific question addressed in the manuscript. Check t...Toward the end of the introduction, often in the last paragraph, it is common to transition from background information to a brief summary of the specific question addressed in the manuscript. Check the boxes to indicate which pieces of information below are in the methods section, and be sure to note the level of detail. The authors state that “Overall, GC-MS demonstrated better precision than HPLC, but the methods had generally equivalent sensitivities.” Consider the data in Tables 1 and 2.
- https://chem.libretexts.org/Courses/Providence_College/CHM_331_Advanced_Analytical_Chemistry_1/14%3A_Liquid_ChromatographyHigh performance liquid chromatography has proven itself to very useful in many scientific fields, yet forces scientists to consistently choose between speed and resolution. Ultra High Performance Liq...High performance liquid chromatography has proven itself to very useful in many scientific fields, yet forces scientists to consistently choose between speed and resolution. Ultra High Performance Liquid Chromatography (UHPLC) eliminates the need to choose and creates a highly efficient method that is primarily based on small particle separations.
