8: Analysis and Separation of Mixtures
- Page ID
- 556148
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\(\newcommand{\avec}{\mathbf a}\) \(\newcommand{\bvec}{\mathbf b}\) \(\newcommand{\cvec}{\mathbf c}\) \(\newcommand{\dvec}{\mathbf d}\) \(\newcommand{\dtil}{\widetilde{\mathbf d}}\) \(\newcommand{\evec}{\mathbf e}\) \(\newcommand{\fvec}{\mathbf f}\) \(\newcommand{\nvec}{\mathbf n}\) \(\newcommand{\pvec}{\mathbf p}\) \(\newcommand{\qvec}{\mathbf q}\) \(\newcommand{\svec}{\mathbf s}\) \(\newcommand{\tvec}{\mathbf t}\) \(\newcommand{\uvec}{\mathbf u}\) \(\newcommand{\vvec}{\mathbf v}\) \(\newcommand{\wvec}{\mathbf w}\) \(\newcommand{\xvec}{\mathbf x}\) \(\newcommand{\yvec}{\mathbf y}\) \(\newcommand{\zvec}{\mathbf z}\) \(\newcommand{\rvec}{\mathbf r}\) \(\newcommand{\mvec}{\mathbf m}\) \(\newcommand{\zerovec}{\mathbf 0}\) \(\newcommand{\onevec}{\mathbf 1}\) \(\newcommand{\real}{\mathbb R}\) \(\newcommand{\twovec}[2]{\left[\begin{array}{r}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\ctwovec}[2]{\left[\begin{array}{c}#1 \\ #2 \end{array}\right]}\) \(\newcommand{\threevec}[3]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\cthreevec}[3]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \end{array}\right]}\) \(\newcommand{\fourvec}[4]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\cfourvec}[4]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \end{array}\right]}\) \(\newcommand{\fivevec}[5]{\left[\begin{array}{r}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\cfivevec}[5]{\left[\begin{array}{c}#1 \\ #2 \\ #3 \\ #4 \\ #5 \\ \end{array}\right]}\) \(\newcommand{\mattwo}[4]{\left[\begin{array}{rr}#1 \amp #2 \\ #3 \amp #4 \\ \end{array}\right]}\) \(\newcommand{\laspan}[1]{\text{Span}\{#1\}}\) \(\newcommand{\bcal}{\cal B}\) \(\newcommand{\ccal}{\cal C}\) \(\newcommand{\scal}{\cal S}\) \(\newcommand{\wcal}{\cal W}\) \(\newcommand{\ecal}{\cal E}\) \(\newcommand{\coords}[2]{\left\{#1\right\}_{#2}}\) \(\newcommand{\gray}[1]{\color{gray}{#1}}\) \(\newcommand{\lgray}[1]{\color{lightgray}{#1}}\) \(\newcommand{\rank}{\operatorname{rank}}\) \(\newcommand{\row}{\text{Row}}\) \(\newcommand{\col}{\text{Col}}\) \(\renewcommand{\row}{\text{Row}}\) \(\newcommand{\nul}{\text{Nul}}\) \(\newcommand{\var}{\text{Var}}\) \(\newcommand{\corr}{\text{corr}}\) \(\newcommand{\len}[1]{\left|#1\right|}\) \(\newcommand{\bbar}{\overline{\bvec}}\) \(\newcommand{\bhat}{\widehat{\bvec}}\) \(\newcommand{\bperp}{\bvec^\perp}\) \(\newcommand{\xhat}{\widehat{\xvec}}\) \(\newcommand{\vhat}{\widehat{\vvec}}\) \(\newcommand{\uhat}{\widehat{\uvec}}\) \(\newcommand{\what}{\widehat{\wvec}}\) \(\newcommand{\Sighat}{\widehat{\Sigma}}\) \(\newcommand{\lt}{<}\) \(\newcommand{\gt}{>}\) \(\newcommand{\amp}{&}\) \(\definecolor{fillinmathshade}{gray}{0.9}\)- 8.1: UV/Vis Analysis of Two-Component Mixtures
- The page discusses the evolution of color matching in spectroscopy, detailing the transition from Nessler's original method to modern photoelectric and infrared methods in the 1930s and 1940s. It then describes different instrument designs for molecular absorption spectroscopy, including filter photometers, single-beam and double-beam spectrophotometers, and diode array spectrometers, highlighting their features and limitations.
- 8.2: Classifying Separation Techniques
- The document details various analytical separation techniques based on differences in chemical or physical properties between analytes and interferents. It includes methods like filtration, dialysis, and chromatography for size-based separations; centrifugation for mass or density differences; masking for complexation reactions; and techniques such as distillation, sublimation, and recrystallization for changes in physical or chemical states.
- 8.3: Liquid-Liquid Extractions
- The document discusses liquid-liquid extraction as a key method for separating compounds, used in environmental, clinical, and industrial labs. It highlights the importance of this technique in monitoring trihalomethanes in water supplies, often through gas chromatography after extraction with pentane. The text explains the concepts of partition coefficients and distribution ratios, emphasizing their roles in determining extraction efficiency.
- 8.4: Overview of Analytical Separations
- In Chapter 7 we examined several methods for separating an analyte from potential interferents. For example, in a liquid–liquid extraction the analyte and interferent initially are present in a single liquid phase. We add a second, immiscible liquid phase and thoroughly mix them by shaking. During this process the analyte and interferents partition between the two phases to different extents, effecting their separation.
- 8.5: General Theory of Column Chromatography
- Of the two methods for bringing the stationary phase and the mobile phases into contact, the most important is column chromatography. In this section we develop a general theory that we may apply to any form of column chromatography.
- 8.6: Optimizing Chromatographic Separations
- Now that we have defined the solute retention factor, selectivity, and column efficiency we are able to consider how they affect the resolution of two closely eluting peaks.
- 8.7: Gas Chromatography
- In gas chromatography (GC) we inject the sample, which may be a gas or a liquid, into an gaseous mobile phase (often called the carrier gas). The mobile phase carries the sample through a packed or a capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase.
- 8.8: High-Performance Liquid Chromatography
- In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase.
- 8.9: Other Forms of Chromatography
- At the beginning of Chapter 12.5, we noted that there are several different types of solute/stationary phase interactions in liquid chromatography, but limited our discussion to liquid–liquid chromatography. In this section we turn our attention to liquid chromatography techniques in which partitioning occurs by liquid–solid adsorption, ion-exchange, and size exclusion.
- 8.10: Electrophoresis
- Electrophoresis is a class of separation techniques in which we separate analytes by their ability to move through a conductive medium—usually an aqueous buffer—in response to an applied electric field. In the absence of other effects, cations migrate toward the electric field’s negatively charged cathode.