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- https://chem.libretexts.org/Courses/California_Polytechnic_State_University_San_Luis_Obispo/Survey_of_Biochemistry_and_Biotechnology/09%3A_Basic_Techniques/9.02%3A_Fractionation_and_Chromatography_TechniquesFractionation of samples, as the name suggests, is a process of separating out the components or fractions of the lysate. Fractionation typically begins with centrifugation of the lysate. Using low-sp...Fractionation of samples, as the name suggests, is a process of separating out the components or fractions of the lysate. Fractionation typically begins with centrifugation of the lysate. Using low-speed centrifugation, one can remove cell debris, leaving a supernatant containing the contents of the cell. By using successively higher centrifugation speeds (and resulting g forces) it is possible to separate out different cellular components, like nuclei, mitochondria, etc., from the cytoplasm.
- https://chem.libretexts.org/Courses/Willamette_University/WU%3A_Chem_199_-_Better_Living_Through_Chemistry/03%3A_Manipulating_Molecules/3.02%3A_Separating_Dissolved_Components/3.2.02%3A_Overview_of_ChromatographyThe first uses of chromatography involved separating the colored components of plants in the early 1900's. The pigments in a plant can be separated into yellow, orange, and green colors (xanthophylls,...The first uses of chromatography involved separating the colored components of plants in the early 1900's. The pigments in a plant can be separated into yellow, orange, and green colors (xanthophylls, carotenes and chlorophylls respectively) through this method. The Greek name for color is chroma, and graphein is 'to write,' so chromatography can be thought of as "color writing."
- https://chem.libretexts.org/Courses/SUNY_Oneonta/Chem_221%3A_Organic_Chemistry_I_(Bennett)/2%3ALab_Textbook_(Nichols)/02%3A_ChromatographyChromatography is a technique used to separate the components of a mixture. It can be used as an analytical technique to gain information about what is present in a mixture, or as a purification tech...Chromatography is a technique used to separate the components of a mixture. It can be used as an analytical technique to gain information about what is present in a mixture, or as a purification technique to separate and collect the components of a mixture.
- https://chem.libretexts.org/Ancillary_Materials/Worksheets/Worksheets%3A_Analytical_Chemistry_II/Selection_of_Appropriate_Separation_MethodPART 1: On the large sheet provided, create a flow chart showing the steps you would take in order to choose a method of separation and detection for a set of analytes. What separation and detection m...PART 1: On the large sheet provided, create a flow chart showing the steps you would take in order to choose a method of separation and detection for a set of analytes. What separation and detection method would you suggest to separate the intact protein insulin from the A and B chains in a mixture of of insulin ? However, the waste water was diverted into a tiny creek running into the river, causing an estimated 1 million gallons of waste water to turn about 80 miles of the river orange.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Analytical_Sciences_Digital_Library/In_Class_Activities/Separation_Science/2%3A_Chromatography__Background/01_IntroductionChromatography refers to a group of methods used as a way of separating mixtures of compounds into their individual components. The basic setup of a chromatographic system involves two phases, one sta...Chromatography refers to a group of methods used as a way of separating mixtures of compounds into their individual components. The basic setup of a chromatographic system involves two phases, one stationary and one mobile. A compound, which we will usually call the solute, is introduced into the system, and essentially has a choice. If it is attracted to the mobile phase, it will move through the system with the mobile phase. If it is attracted to the stationary phase, it will lag behind.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.10%3A_Chapter_Summary_and_Key_TermsThis page highlights the fundamentals of chromatography and electrophoresis, potent analytical techniques used to separate and analyze sample components. It details concepts such as retention factors,...This page highlights the fundamentals of chromatography and electrophoresis, potent analytical techniques used to separate and analyze sample components. It details concepts such as retention factors, column efficiency, and selectivity in chromatographic separations, and how methods like gas chromatography (GC) and high-performance liquid chromatography (HPLC) are applied.
- https://chem.libretexts.org/Courses/Sewanee%3A_The_University_of_the_South/Organic_Chemistry_Lab_Textbook/09%3A_Chromatography/9.02%3A_Chromatography_Generalities/9.2A%3A_Overview_of_ChromatographyThe first uses of chromatography involved separating the colored components of plants in the early 1900's. The pigments in a plant can be separated into yellow, orange, and green colors (xanthophylls,...The first uses of chromatography involved separating the colored components of plants in the early 1900's. The pigments in a plant can be separated into yellow, orange, and green colors (xanthophylls, carotenes and chlorophylls respectively) through this method. The Greek name for color is chroma, and graphein is 'to write,' so chromatography can be thought of as "color writing."
- https://chem.libretexts.org/Courses/Sewanee%3A_The_University_of_the_South/Organic_Chemistry_Lab_Textbook/09%3A_Chromatography/9.02%3A_Chromatography_GeneralitiesChromatography in the organic chemistry laboratory can be classified into several broad categories: Thin-lay chromatography, Column chromatography and gas chromatography. These techniques follow the s...Chromatography in the organic chemistry laboratory can be classified into several broad categories: Thin-lay chromatography, Column chromatography and gas chromatography. These techniques follow the same general principles in terms of how they are able to separate mixtures, and so will be discussed collectively in this section. Details specific to each technique will then be further discussed in their own sections.
- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_(OpenStax)/26%3A_Biomolecules-_Amino_Acids_Peptides_and_Proteins/26.05%3A_Amino_Acid_Analysis_of_PeptidesThis section discusses methods for analyzing amino acids in peptides, focusing on techniques like chromatography and mass spectrometry. It highlights the importance of these methods in determining pep...This section discusses methods for analyzing amino acids in peptides, focusing on techniques like chromatography and mass spectrometry. It highlights the importance of these methods in determining peptide composition and structure, which are crucial for understanding biological functions. The analysis aids in identifying the sequence of amino acids and can reveal modifications that influence peptide behavior.
- https://chem.libretexts.org/Bookshelves/General_Chemistry/Chem1_(Lower)/02%3A_Essential_Background/2.01%3A_Classification_and_Properties_of_MatterMatter is “anything that has mass and occupies space”. Matter is what chemical substances are composed of. But what do we mean by chemical substances? How do we organize our view of matter and its pro...Matter is “anything that has mass and occupies space”. Matter is what chemical substances are composed of. But what do we mean by chemical substances? How do we organize our view of matter and its properties? These very practical questions will be the subjects of this lesson.
- https://chem.libretexts.org/Workbench/Pick_Your_Poison%3A_Introduction_to_Materials_Toxicology/25%3A_Biomolecules-_Amino_Acids_Peptides_and_Proteins/25.06%3A_Amino_Acid_Analysis_of_PeptidesThis section discusses methods for analyzing amino acids in peptides, focusing on techniques like chromatography and mass spectrometry. It highlights the importance of these methods in determining pep...This section discusses methods for analyzing amino acids in peptides, focusing on techniques like chromatography and mass spectrometry. It highlights the importance of these methods in determining peptide composition and structure, which are crucial for understanding biological functions. The analysis aids in identifying the sequence of amino acids and can reveal modifications that influence peptide behavior.
