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7.2: Using Calibrated Glass Pipettes

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    Table 7.2: Procedural summary for using calibrated glass pipettes.

    Place pipette tip in reagent bottle, squeeze pipette bulb, and connect to the pipette.

    Partially release your hand to create suction. Do not let to completely or liquid will withdraw forcibly and possibly into the bulb.

    Apply suction until liquid is withdrawn to just past the desired mark.

    Remove the pipette bulb and place your finger atop the pipette.

    Allow tiny amounts of air to be let into the top of the pipette by wiggling your finger or a slight release of pressure.

    Drain the liquid to the desired mark.

    Tightly hold the pipette with your finger, bring it to the transfer flask and deliver the reagent to the desired mark.

    Touch the pipette to the side of the container to dislodge the drip at the end of the pipette.

    If a pipette is drained to the tip,

    • To-deliver (T.D.) pipettes and volumetric pipettes should not be blown out.
    • To-contain (T.C.) pipettes should be "blown out".
     Note: If pipette is wet with a different solution before use, obtain a fresh one or "condition" the pipette with two rinses of the reagent.


    This page titled 7.2: Using Calibrated Glass Pipettes is shared under a CC BY-NC-ND 4.0 license and was authored, remixed, and/or curated by Lisa Nichols via source content that was edited to the style and standards of the LibreTexts platform; a detailed edit history is available upon request.