Search
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.10%3A_Chapter_Summary_and_Key_TermsThis page highlights the fundamentals of chromatography and electrophoresis, potent analytical techniques used to separate and analyze sample components. It details concepts such as retention factors,...This page highlights the fundamentals of chromatography and electrophoresis, potent analytical techniques used to separate and analyze sample components. It details concepts such as retention factors, column efficiency, and selectivity in chromatographic separations, and how methods like gas chromatography (GC) and high-performance liquid chromatography (HPLC) are applied.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.02%3A_General_Theory_of_Column_ChromatographyThis page provides a comprehensive overview of column chromatography, focusing on the theoretical concepts and calculations essential for understanding this separation technique. It introduces key con...This page provides a comprehensive overview of column chromatography, focusing on the theoretical concepts and calculations essential for understanding this separation technique. It introduces key concepts like chromatographic resolution, retention time, selectivity, and column efficiency. The content explains how solutes are separated and how to analyze chromatographic data to assess separation quality.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/26%3A_Introduction_to_Chromatographic_Separations/26.01%3A_A_General_Description_of_ChromatographyIn chromatography we pass a sample-free phase, which we call the mobile phase, over a second sample-free stationary phase that remains fixed in space. We inject the sample into the mobile phase where ...In chromatography we pass a sample-free phase, which we call the mobile phase, over a second sample-free stationary phase that remains fixed in space. We inject the sample into the mobile phase where its components partition between the mobile phase and the stationary phase. The types of mobile phases and stationary phases, how these two phases contact each other, and how the solutes interact with the two phases are useful ways describe a chromatographic method.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/26%3A_Introduction_to_Chromatographic_SeparationsDespite the power of these instrumental methods of analysis, their use is often limited if the sample contains species that will interfere with the analysis. Chromatography provides a solution to the ...Despite the power of these instrumental methods of analysis, their use is often limited if the sample contains species that will interfere with the analysis. Chromatography provides a solution to the analysis of complex samples by providing a way to separate the individual species in a sample prior to their analysis by a spectroscopic or electroanalytical method of analysis.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/28%3A_High-Performance_Liquid_Chromatography/28.05%3A_Adsorption_ChromatographyIn adsorption chromatography (or liquid-solid chromatography, LSC) the column packing also serves as the stationary phase. For most samples, liquid–solid chromatography does not offer any special adva...In adsorption chromatography (or liquid-solid chromatography, LSC) the column packing also serves as the stationary phase. For most samples, liquid–solid chromatography does not offer any special advantages over liquid–liquid chromatography. One exception is the analysis of isomers, where LSC excels.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/28%3A_High-Performance_Liquid_ChromatographyIn high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column t...In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase. By adjusting conditions it usually is possible to design a separation so that analytes elute by themselves, even when the mixture is complex.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.05%3A_High-Performance_Liquid_ChromatographyThis page discusses High-Performance Liquid Chromatography (HPLC), a method for separating compounds in a liquid solution. HPLC involves key components: reservoirs for the mobile phase, a pump, an inj...This page discusses High-Performance Liquid Chromatography (HPLC), a method for separating compounds in a liquid solution. HPLC involves key components: reservoirs for the mobile phase, a pump, an injector, columns (analytical and guard), and detectors. It explains different separation techniques (liquid-solid adsorption, liquid-liquid partitioning, etc.), types of columns (packed and capillary), stationary and mobile phases, and their interactions.
- https://chem.libretexts.org/Courses/Martin_Luther_College/Organic_Chemistry_-_MLC/02%3A_Tools_of_Organic_Chemistry/2.01%3A_Intermolecular_Forces_(IMFs)_-_Review/2.1.03%3A_ChromatographyChromatography is an efficient way for chemists to separate and analyze mixtures. Read on to find out how this critical process works.
- https://chem.libretexts.org/Courses/University_of_North_Texas/UNT%3A_CHEM_1410_-_General_Chemistry_for_Science_Majors_I/Text/10%3A_Solids_Liquids_and_Solutions/10.23%3A_ChromatographyChromatography is an efficient way for chemists to separate and analyze mixtures. Read on to find out how this critical process works.
- https://chem.libretexts.org/Bookshelves/General_Chemistry/ChemPRIME_(Moore_et_al.)/10%3A_Solids_Liquids_and_Solutions/10.23%3A_ChromatographyChromatography is an efficient way for chemists to separate and analyze mixtures. Read on to find out how this critical process works.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.04%3A_Gas_ChromatographyGas chromatography (GC) is a technique in which a sample, either gas or liquid, is injected into a mobile phase, usually a chemically inert gas like He, Ar, or N2, which carries it through a column. I...Gas chromatography (GC) is a technique in which a sample, either gas or liquid, is injected into a mobile phase, usually a chemically inert gas like He, Ar, or N2, which carries it through a column. In a packed column, larger samples can be handled, whereas capillary columns allow separation of more complex mixtures. Elution order in gas-liquid chromatography depends on solutes' boiling points and interactions with the stationary phase.
