8.3: Procedures
- Page ID
- 374956
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Experimental Procedures:
- Fill 50 mL burette with 0.005 M ferrous iron.
- Fill 50 mL burette with 0.005 M unknown ligand.
- Label fifteen 50 mL beakers with the mole fractions of iron as in the data sheet.
- Transfer to labeled beakers the proper amount of solution. Each beaker should contain 10 mL of total solution.
- Wait at least 20 minutes for the reaction to complete.
- Choose wavelength for absorbance measurements.
- Zero out the spectrometer with a blank solution of water.
- Take a spectra of the darkest solution.
- Choose a wavelength where the darkest solution approaches 1.
- Write this wavelength down on the data sheet.
- Record the absorbance of each solution in data sheet at the wavelength chosen in step 6.
- Double check that the reaction is over by checking the solutions that represent stoichiometric proportions of the hypothetical complexes to see if the absorbance has increased. Once all solutions maintain a constant value you can work up the data.
