- 12.1: Overview of Analytical Separations
- In Chapter 7 we examined several methods for separating an analyte from potential interferents. For example, in a liquid–liquid extraction the analyte and interferent initially are present in a single liquid phase. We add a second, immiscible liquid phase and thoroughly mix them by shaking. During this process the analyte and interferents partition between the two phases to different extents, effecting their separation.
- 12.2: General Theory of Column Chromatography
- Of the two methods for bringing the stationary phase and the mobile phases into contact, the most important is column chromatography. In this section we develop a general theory that we may apply to any form of column chromatography.
- 12.3: Optimizing Chromatographic Separations
- Now that we have defined the solute retention factor, selectivity, and column efficiency we are able to consider how they affect the resolution of two closely eluting peaks.
- 12.4: Problems
- End-of-chapter problems to test your understanding of topics covered in this chapter.
Thumbnail: Separation of black ink on a thin layer chromatography plate. Image used with permission (CC BY-SA 3.0; Natrij).