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12: An Introduction to Chromatographic Separations

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    • 12.1: Overview of Analytical Separations
      In Chapter 7 we examined several methods for separating an analyte from potential interferents. For example, in a liquid–liquid extraction the analyte and interferent initially are present in a single liquid phase. We add a second, immiscible liquid phase and thoroughly mix them by shaking. During this process the analyte and interferents partition between the two phases to different extents, effecting their separation.
    • 12.2: General Theory of Column Chromatography
      Of the two methods for bringing the stationary phase and the mobile phases into contact, the most important is column chromatography. In this section we develop a general theory that we may apply to any form of column chromatography.
    • 12.3: Optimizing Chromatographic Separations
      Now that we have defined the solute retention factor, selectivity, and column efficiency we are able to consider how they affect the resolution of two closely eluting peaks.
    • 12.4: Problems
      End-of-chapter problems to test your understanding of topics covered in this chapter.

    Thumbnail: Separation of black ink on a thin layer chromatography plate. Image used with permission (CC BY-SA 3.0; Natrij).

    12: An Introduction to Chromatographic Separations is shared under a not declared license and was authored, remixed, and/or curated by LibreTexts.

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