General Reference: http://www.prenhall.com/settle/chapters/ch26.pdf
- Know the possible deactivation processes available to a molecule which absorbs a photon and undergoes an electronic energy transition.
- Be able to explain the impact of molecular structure, temperature, solvent, and concentration on fluorescence.
http://www.springerlink.com/content/qp463n1w010033h7/fulltext.pdf -- solvent effects
- Be able to explain the various instrumental components and diagrams for fluorimeters and spectrofluorimeters.
http://www.fluorescence-foundation.org/lectures/genova2006/lecture3.ppt (This site asks for user name and password, but opens without them when “cancel” is selected)
- Be able to acquire and process data from an analysis. This includes, but is not limited to (quantitative analysis): obtaining a signal for a blank, standards, and unknown; taking into account how the sample was processed (sample blank), which includes dilutions of both the sample and standards; plotting the data (Signal vs. Concentration) and obtaining a calibration curve; calculating a limit of detection for the analyte.
Harvey 10, end of chapter problems. 40-43