# 7.11: Report on the Nitrogenase Crystal Structure$$\;^{378-381}$$

A significant breakthrough has occurred in the crystallographic analysis of the iron-molybdenum protein of nitrogenase. The overall distribution of the metal clusters in the protein is shown in Figure 7.40. The distance between the two FeMoco units is fully consistent with each cofactor acting as an independent active site. On the other hand, the closeness of the P cluster and FeMoco centers in each unit is indicative of their likely cooperation in the N2 fixation reaction.

The proposed structure of the P cluster, shown in Figure 7.41, involves a doubly bridged, double cubane unit consisting of one normally bound Fe4S4 cluster with all cysteine ligands and one Fe4S4 cluster that contains an unusual cysteine/serine (S/O) ligand pair on one of its two nonbridged Fe positions. Such five-coordinate iron in an Fe4S4 cluster is not unprecedented.138 The two Fe4S4 clusters are disposed to produce a face-sharing arrangement with two cysteine ligands bridging the two sets of Fe atoms. An interesting feature of the structure is a disulfide unit linking the two clusters; this unit potentially could be redox-active during nitrogenase turnover.

Most striking of the new results is the proposed structure of FeMoco shown in Figure 7.42. The cluster core of composition Fe7MoS8 can be viewed as two halves bridged by two S2- ions and an unknown ligand (designated Y in the figure). The MoFe3S3 half of the core is in the shape of a thiocubane fragment missing one $$\mu_{3}$$-S2- ion. The Mo is six coordinate; the ligands are three $$\mu_{2}$$-S2- ions, which bridge to the three Fe ions, an $$\alpha$$-His-442 nitrogen, and two oxygen donors (the hydroxyl and central carboxylate) of the homocitrate ligand. Interestingly, the second half of FeMoco is a similar thiocubane fragment, Fe4S3, also missing a $$\mu_{3}$$-S2- ion. This unit has a single noncore ligand, $$\alpha$$-Cys-275, which is bound to the terminal Fe atom of the cluster. The two thiocubane fragments (MoFe3S3 and Fe4S3) are bridged by three ligands in a face-sharing mode with the two Fe3 faces eclipsed with respect to each other. The eight metal ions display a bis(end-capped) trigonal prismatic arrangement with three bridges on the edges of the prism, which connect the two thiocubane fragments. The two sulfide bridges between the thiocubane halves are clearly defined in the structure, but the third bridge is not, suggesting the possibility that this is in fact part of the N2-binding site. Interestingly, $$\alpha$$-His-195, identified as essential for N2 fixation by mutagenesis and ESEEM studies, does not appear to be covalently bound, although it is close to the FeMoco unit.

Clearly, this structure is not the same as any of those previously proposed (Figure 7.31), although it does possess many features that were identified in model studies. While it is tempting to speculate that the central bridge of the cluster (the Y ligand) is the site of N2 reduction, this is in no way established at present. The structural definition of the nitrogenase proteins is now progressing at a rapid rate. Many of the physical measurements will have to be reexamined in light of the new data. Through further experimentation involving physical methods, mutagenesis, and kinetic/mechanistic studies, much more information about the role of ATP, the activation of hydrogen, and the binding, activation, and reduction of N2 and other nitrogenase substrates should be obtained.