18g: Summary
- Page ID
- 150642
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Name: ______________________________
Section: _____________________________
Student ID#:__________________________
Summary
Schematics for Oxidative Phosphorylation:
- Complete the following picture of oxidative phosphorylation with the following pieces of information:
- Indicate where the TCA cycle occurs.
- Show the flow of protons for each complex.
- Indicate the source of electrons into Complex 1.
- Indicate the reaction that is the sink of eletrons for Complex IV.
- Draw in the mobile e-carries (cyt c and UQ) and their paths.
- For each mobile reactant/ product, draw their Lewis structure (for cytochrome C deaw the heme) .
- Show where ATP is produced.
- Write the balanced net equation for each complex.
- Calculate the ΔG for each individual complex.
Practice Problems:
- Matching: Choose the correct answer from the list below. Not all of the answers will be used.
- mitochondria
- FMN
- iron-sulfur clusters
- transporters
- succinate dehydrogenase
- cytochrome c oxidase (or Complex IV)
- coenzyme Q (or UQ)
____________ This is where oxidative phosphorylation occurs in eukaryotes.
____________ This electron carrier is a derivative of quinone and has an isoprenoid tail.
____________ This enzyme catalyzes the reduction of O2.
____________ This prosthetic group is present in complexes I, II, and III of electron transport.
____________ This citric acid cycle enzyme is also part of an electron-transport complex.
- Fill in the blank:
- A strong oxidizing agent has a strong tendency to ____________ (accept, donate) electron(s).
- In the initial step of Complex I, two high-potential electrons are transferred from NADH to the ___________ prosthetic group of this complex.
- Cytochrome ________ is the only water-soluble cytochrome of the electron-transport chain.
- Cytochrome c oxidase contains two heme A groups and three ______________ ions.
- ________________ carries electrons from Complex III to Complex IV.
- _________________ is a poison because it blocks the flow of electrons from cytochrome c to oxygen.
- Match complex I-V with its characteristics (there may be more than one answer per line).
- pumps protons across membrane
- takes part in electron chain
- sends out ubiquinol as a mobile electron carrier
- sends out cytochrome c as a mobile electron carrier
- reduces O2 to H2O
- produces ATP
- Julia Janzon and coworkers at Goethe University in Frankfurt studied electron transfer from mobile cytochrome c1F to ruthenium-substituted cytochrome c522F. The ionic strength of the solution was increased by adding KCl.
- What happens to the electron transfer rate constant (k12) as ionic strength (I) increases?
- Propose a physical reason for this relationship.
- In mutagenesis studies, replacement of a glutamic acid near the heme in c522F with a lysine cuts the electron transfer rate in half. Suggest an explanation for this observation.