Drawing from an arsenal of analytical techniques—many of which were the subject of the preceding four chapters—analytical chemists design methods for the analysis of analytes at increasingly lower concentrations and in increasingly more complex matrices. Despite the power of these analytical techniques, they often suffer from a lack of selectivity. For this reason, many analytical procedures include a step to separate the analyte from potential interferents. Although effective, each additional step in an analytical procedure increases the analysis time and introduces uncertainty. In this chapter we consider two analytical techniques that avoid these limitations by combining the separation and analysis: chromatography and electrophoresis.
Thumbnail: Separation of black ink on a thin layer chromatography plate. Image used with permission (CC BY-SA 3.0; Natrij)}