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- https://chem.libretexts.org/Courses/can/CHEM_232_-_Organic_Chemistry_II_(Puenzo)/14%3A_Biomolecules_-_Nucleic_Acids/14.07%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Courses/Martin_Luther_College/Organic_Chemistry_-_MLC/05%3A_Biomolecules/5.04%3A_Biomolecules_-_Nucleic_Acids/5.4.06%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Courses/Williams_School/Chemistry_II/09%3A_Biomolecules_-_Nucleic_Acids/9.06%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Workbench/LCDS_Organic_Chemistry_OER_Textbook_-_Todd_Trout/28%3A_Biomolecules_-_Nucleic_Acids/28.07%3A_DNA_SequencingThis product mixture is then separated according to the size of the pieces by gel electrophoresis (Section 26.2), and the identity of the terminal dideoxyribonucleotide in each piece—and thus the sequ...This product mixture is then separated according to the size of the pieces by gel electrophoresis (Section 26.2), and the identity of the terminal dideoxyribonucleotide in each piece—and thus the sequence of the restriction fragment—is determined by noting the color with which the attached dye fluoresces.
- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_III_(Morsch_et_al.)/28%3A_Nucleic_Acids/28.06%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Courses/Brevard_College/CHE_301_Biochemistry/06%3A_Nucleic_Acids/6.15%3A_DNA_SequencingIf a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.)...If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Courses/Smith_College/Organic_Chemistry_(LibreTexts)/28%3A_Biomolecules_-_Nucleic_Acids/28.06%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_(OpenStax)/28%3A_Biomolecules_-_Nucleic_Acids/28.06%3A_DNA_SequencingThis product mixture is then separated according to the size of the pieces by gel electrophoresis (Section 26.2), and the identity of the terminal dideoxyribonucleotide in each piece—and thus the sequ...This product mixture is then separated according to the size of the pieces by gel electrophoresis (Section 26.2), and the identity of the terminal dideoxyribonucleotide in each piece—and thus the sequence of the restriction fragment—is determined by noting the color with which the attached dye fluoresces.
