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- https://chem.libretexts.org/Courses/Los_Angeles_Trade_Technical_College/Analytical_Chemistry/2%3A_Analytical_Chemistry_2.0_(Harvey)/13%3A_Chromatographic/13.3%3A_Optimizing_Chromatographic_SeparationsNow that we have defined the solute retention factor, selectivity, and column efficiency we are able to consider how they affect the resolution of two closely eluting peaks. Because the two peaks have...Now that we have defined the solute retention factor, selectivity, and column efficiency we are able to consider how they affect the resolution of two closely eluting peaks. Because the two peaks have similar retention times, it is reasonable to assume that their peak widths are nearly identical.
- https://chem.libretexts.org/Ancillary_Materials/Demos_Techniques_and_Experiments/General_Lab_Techniques/Thin_Layer_ChromatographyThin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. It may be performed on the anal...Thin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. It may be performed on the analytical scale as a means of monitoring the progress of a reaction, or on the preparative scale to purify small amounts of a compound. TLC is an analytical tool widely used because of its simplicity, relative low cost, high sensitivity, and speed of separation.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.10%3A_Chapter_Summary_and_Key_TermsThis page highlights the fundamentals of chromatography and electrophoresis, potent analytical techniques used to separate and analyze sample components. It details concepts such as retention factors,...This page highlights the fundamentals of chromatography and electrophoresis, potent analytical techniques used to separate and analyze sample components. It details concepts such as retention factors, column efficiency, and selectivity in chromatographic separations, and how methods like gas chromatography (GC) and high-performance liquid chromatography (HPLC) are applied.
- https://chem.libretexts.org/Courses/Los_Angeles_Trade_Technical_College/Analytical_Chemistry/2%3A_Analytical_Chemistry_2.0_(Harvey)/13%3A_Chromatographic/13.2%3A_General_Theory_of_Column_ChromatographyOf the two methods for bringing the stationary phase and the mobile phases into contact, the most important is column chromatography. In this section we develop a general theory that we may apply to a...Of the two methods for bringing the stationary phase and the mobile phases into contact, the most important is column chromatography. In this section we develop a general theory that we may apply to any form of column chromatography.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/26%3A_Introduction_to_Chromatographic_Separations/26.02%3A_Migration_Rates_of_SolutesOur ability to separate two solutes depends on the equilibrium interactions of the solute with the stationary phase and the mobile phase, which effects both the time it takes a solute to travel throug...Our ability to separate two solutes depends on the equilibrium interactions of the solute with the stationary phase and the mobile phase, which effects both the time it takes a solute to travel through the column and how the width of the solute's elution profile. In this section we consider the rate at which the solute moves through the column.
- https://chem.libretexts.org/Courses/BethuneCookman_University/B-CU%3A_CH-345_Quantitative_Analysis/CH345_Labs/Demonstrations_and_Techniques/General_Lab_Techniques/Thin_Layer_ChromatographyThin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. It may be performed on the anal...Thin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. It may be performed on the analytical scale as a means of monitoring the progress of a reaction, or on the preparative scale to purify small amounts of a compound. TLC is an analytical tool widely used because of its simplicity, relative low cost, high sensitivity, and speed of separation.
