Triple resonance experiments (3D) are a set of multi-dimensional nuclear magnetic resonance spectroscopy (NMR) experiments that link three types of atomic nuclei, typically consisting of 1H, 15N and 1...Triple resonance experiments (3D) are a set of multi-dimensional nuclear magnetic resonance spectroscopy (NMR) experiments that link three types of atomic nuclei, typically consisting of 1H, 15N and 13C. These experiments are often used to assign specific resonance signals to specific atoms in an isotopically enriched protein.
In the previous Chapter we described 2D NMR spectroscopy, which offers significantly greater spectral resolution than basic 1D spectra. In this Chapter we will show how the well-resolved 2D 15N-HSQC r...In the previous Chapter we described 2D NMR spectroscopy, which offers significantly greater spectral resolution than basic 1D spectra. In this Chapter we will show how the well-resolved 2D 15N-HSQC resonances can be assigned to specific residues and chemical groups within protein samples. As an example, we will consider a couple of complementary types of 3D NMR data: HNCACB and CBCA(CO)NH and their joint application for making heteronuclear NMR resonance assignment in proteins.?? Such an assign
