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7.1: 2D NMR

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    • 2D NMR: Indirect Detection
      The reverse detection technique became increasingly popular due to the software improvements of the spectrometers from one hand and to the increase of the sensitivity of the detection of a X nucleus by the indirect way. In fact the reverse detection solves the problem of the low concentrations. Moreover, it allows to reach the NMR parameters such as chemical shifts, coupling constants and relaxation time spin lattice for nuclei impossible to study by the direct detection.
    • 2D NMR Background
      These atoms and some others behave, basically, as small magnets with a so called nuclear magnetic momentum quantified by a quantic value: the spin.
    • 2D NMR Basics
      The 2D NMR experiment belongs as well to the Fourier transform spectroscopy than to the impulsion one and relies on a sequence of three time intervals: preparation, evolution and detection (3). In some experiment another time interval is added before the detection: the mixing time.
    • 2D NMR Experiments
      An overview of 2D NMR fundamentals is given.
    • 2D NMR Introduction
      Some general principles and techniques used in two-dimensional NMR are discussed. Applications covered are mostly concerned with protein NMR, but additional 2D techniques and applications can be found in the references section.
    • Heteronuclear Correlations
      The heteronuclear correlation of chemical shifts by scalar coupling derives from the existence of heteronuclear scalar coupling all owing a magnetization transfer from the more sensitive nucleus (1H) toward the less sensitive nucleus (13C).
    • Homonuclear Correlations
      This sequence allows to get the coupling constant along the Fl axis and the chemical shift of each uncoupled proton along the F2 axis (Spectrum 1 and 2). Of course, the couplings involving two different nuclei (31P, 19P coupled to 1H) are always found in the F2 dimension. One can thus determine the coupling between a proton within a multiplet of a coupled spectrum and phosphorus.

    7.1: 2D NMR is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Marc Bria.

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