Students will handle a number of chemicals during this experiment, many of which must be treated with care in order to avoid harm. None of the chemicals listed here should be ingested or allowed to come in contact with your skin or eyes. The TAs will provide additional safety information and procedures during the weekly pre-laboratory lectures for this module.
- Sodium dihydrogen phosphate monohydrate: An irritant. Target organs include eyes, skin and respiratory tract. Can produce delayed pulmonary edema.
- Catalase from bovine liver culture: Classified as non-hazardous. However, do not breathe the dust. Treat it as a potential biological hazard. Incompatible with strong oxidizing agents. Air and moisture sensitive.
- Bovine Serum Albumin (BSA): No dangerous reactions known. No adverse health effects. Large doses may cause gastro-intestinal upset.
- MOPS Buffer: May be harmful if swallowed, inhaled or absorbed through the skin. May cause irritation to skin, eyes, and respiratory tract.
- Coomassie® Dye: Potential and acute chronic health effects are unknown. Avoid contact with eyes, skin and clothing. No known significant hazards.
- 30% Hydrogen Peroxide: A dangerous strong oxidizer. Contact with other material may cause fire. Corrosive. Causes severe burns to skin, eyes and respiratory tract. Harmful if swallowed or inhaled. Use gloves when pouring.
- Tris HCl Buffer: May cause irritation to the respiratory tract. Symptoms may include coughing and shortness of breath. Skin irritant producing redness and pain. Chronic dermatitis may follow skin contact.
8 Various Material Safety Data Sheets: Malinckrodt Chemicals, J. T. Baker, Phillipsburg, NJ; Fischer Scientific, MSDS, Pittsburgh, PA