1.41: Molecular Biology in the Clinical Laboratory
- Page ID
- 125354
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- Complementary DNA (cDNA) is best defined as:
- two DNA strands, each strand consisting of one type of purine base
- two DNA strands, each strand consisting of one type of pyrimidine base
- two DNA strands, covalently linked to each other
- a strand of DNA in which the order of pyrimidine and purine bases complement the opposite base order on a strand of RNA
- a strand of DNA in which the order of pyrimidine and purine bases complement the opposite base order on a strand of DNA
- The polymerase chain reaction (PCR) is a technique used to:
- increase the sensitivity of enzyme reactions
- enhance the sensitivity of DNA detection methods
- cross-ink bonded stationary phases for HPLC
- measure polymerized collagen in urine
- degrade DNA to smaller fragments
- Analysis of DNA fragments by complementary DNA hybridization is performed by:
- Northern blot technique
- Southern blot technique
- Western blot technique
- Sandwich technique
- ELISA technique
Use the following Key to answer Questions 4-5.
- 1, 2,and 3 are correct
- 1 and 3 are correct
- 2 and 4 are correct
- only 4 is correct
- all are correct
- Genetic defects can be detected in vitro by:
- analysis of DNA
- measuring amniotic fluid lecithin and sphingomyelin
- measurement of specific enzyme levels
- measurement of amniotic fluid bilirubin
- Restriction enzymes are:
- a group of bacterial enzymes
- enzymes that inhibit DNA replication
- enzymes that “cut” (hydrolyze) at specific base sequences
- a group of enzymes that are involved with keeping specific ions outside of the cell.
- Answer
-
- e (p. 938)
- b (p. 940-941)
- b (p. 940941)
- b (p. 939)
- b (p. 939)