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- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.10%3A_Chapter_Summary_and_Key_TermsThis page highlights the fundamentals of chromatography and electrophoresis, potent analytical techniques used to separate and analyze sample components. It details concepts such as retention factors,...This page highlights the fundamentals of chromatography and electrophoresis, potent analytical techniques used to separate and analyze sample components. It details concepts such as retention factors, column efficiency, and selectivity in chromatographic separations, and how methods like gas chromatography (GC) and high-performance liquid chromatography (HPLC) are applied.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/28%3A_High-Performance_Liquid_Chromatography/28.01%3A_Scope_of_HPLCHigh-performance liquid chromatography consists of four broad types of separations: the partitioning of a solute between two liquid phases, adsorption of a solute on a solid substrate, the attraction ...High-performance liquid chromatography consists of four broad types of separations: the partitioning of a solute between two liquid phases, adsorption of a solute on a solid substrate, the attraction of an ionic solute to an ion-exchange resin, and the exclusion of a sufficiently large solute from entering into a solid substrate.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.05%3A_High-Performance_Liquid_ChromatographyThis page discusses High-Performance Liquid Chromatography (HPLC), a method for separating compounds in a liquid solution. HPLC involves key components: reservoirs for the mobile phase, a pump, an inj...This page discusses High-Performance Liquid Chromatography (HPLC), a method for separating compounds in a liquid solution. HPLC involves key components: reservoirs for the mobile phase, a pump, an injector, columns (analytical and guard), and detectors. It explains different separation techniques (liquid-solid adsorption, liquid-liquid partitioning, etc.), types of columns (packed and capillary), stationary and mobile phases, and their interactions.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.09%3A_Additional_ResourcesThe page provides a comprehensive list of experiments and references related to chromatography and electrophoresis. It categorizes experiments into gas chromatography, high-performance liquid chromato...The page provides a comprehensive list of experiments and references related to chromatography and electrophoresis. It categorizes experiments into gas chromatography, high-performance liquid chromatography, ion-exchange chromatography, size-exclusion chromatography, and electrophoresis.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/34%3A_Particle_Size_DeterminationA typical packed column used porous silica particles with a mean diameter between 3-10 µm in size. A column that a manufacturer advertises as using 5 µm particles, however, will contain particles that...A typical packed column used porous silica particles with a mean diameter between 3-10 µm in size. A column that a manufacturer advertises as using 5 µm particles, however, will contain particles that are smaller and particles that are larger. As the size of the particles has an effect on the pressure it takes to move the mobile phase through the column, knowledge of the distribution of particle sizes is of interest. In this chapter we consider different methods for determining particle size.
- https://chem.libretexts.org/Courses/Los_Angeles_Trade_Technical_College/Analytical_Chemistry/2%3A_Analytical_Chemistry_2.0_(Harvey)/13%3A_Chromatographic/13.5%3A_High-Performance_Liquid_ChromatographyIn high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column t...In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase.
