This chapter focuses on flow injection analysis (FIA), a technique introduced in the mid-1970s for automated sample analysis. FIA involves injecting samples into a flowing carrier stream, leading to t...This chapter focuses on flow injection analysis (FIA), a technique introduced in the mid-1970s for automated sample analysis. FIA involves injecting samples into a flowing carrier stream, leading to transient signals at a detector, influenced by physical and chemical processes. The chapter outlines FIA's theory, describing components like propelling units, injectors, detectors, and transport systems. Applications span environmental and clinical analyses.
The text covers various liquid chromatography techniques, focusing on liquid-solid adsorption, ion-exchange, and size-exclusion chromatography. Liquid-solid chromatography involves polar stationary ph...The text covers various liquid chromatography techniques, focusing on liquid-solid adsorption, ion-exchange, and size-exclusion chromatography. Liquid-solid chromatography involves polar stationary phases and nonpolar solvents for separating compounds, excelling in analyzing isomers. Ion-exchange chromatography uses resin beads with ionic functional groups to separate ions based on their affinity for the exchange sites, utilizing gradient elutions to affect solute retention.
