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- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_(Morsch_et_al.)/28%3A_Biomolecules_-_Nucleic_Acids/28.06%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Bookshelves/Introductory_Chemistry/Introductory_Chemistry_(CK-12)/26%3A_Biochemistry/26.13%3A_Genetic_CodeThis page summarizes the Human Genome Project, which aimed to sequence human DNA and was completed in April 2003. It discusses the role of DNA in determining protein production through genes, the gene...This page summarizes the Human Genome Project, which aimed to sequence human DNA and was completed in April 2003. It discusses the role of DNA in determining protein production through genes, the genetic code's structure, and the significance of unique DNA sequences in heredity and biodiversity.
- https://chem.libretexts.org/Courses/can/CHEM_232_-_Organic_Chemistry_II_(Puenzo)/14%3A_Biomolecules_-_Nucleic_Acids/14.07%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Workbench/Pick_Your_Poison%3A_Introduction_to_Materials_Toxicology/26%3A_Biomolecules_-_Nucleic_Acids/26.07%3A_DNA_SequencingDNA sequencing involves determining the order of nucleotides in DNA. Key methods include Sanger sequencing, which uses chain-terminating nucleotides for small-scale projects, and next-generation seque...DNA sequencing involves determining the order of nucleotides in DNA. Key methods include Sanger sequencing, which uses chain-terminating nucleotides for small-scale projects, and next-generation sequencing (NGS), which enables rapid and extensive sequencing of entire genomes and has revolutionized genomics, allowing for advancements in medical research, personalized medicine, and evolutionary studies. These techniques provide insights into genetic variation and the role of genes in health.
- https://chem.libretexts.org/Courses/Martin_Luther_College/Organic_Chemistry_-_MLC/05%3A_Biomolecules/5.04%3A_Biomolecules_-_Nucleic_Acids/5.4.06%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Workbench/LCDS_Organic_Chemistry_OER_Textbook_-_Todd_Trout/28%3A_Biomolecules_-_Nucleic_Acids/28.07%3A_DNA_SequencingThis product mixture is then separated according to the size of the pieces by gel electrophoresis (Section 26.2), and the identity of the terminal dideoxyribonucleotide in each piece—and thus the sequ...This product mixture is then separated according to the size of the pieces by gel electrophoresis (Section 26.2), and the identity of the terminal dideoxyribonucleotide in each piece—and thus the sequence of the restriction fragment—is determined by noting the color with which the attached dye fluoresces.
- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_III_(Morsch_et_al.)/28%3A_Nucleic_Acids/28.06%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Courses/Smith_College/Organic_Chemistry_(LibreTexts)/28%3A_Biomolecules_-_Nucleic_Acids/28.06%3A_DNA_SequencingThe computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction m...The computer also keeps track of the order in which the terminating nucleotides appeared, which is the sequence of the DNA used in the original reaction. If a different dye is used for each reaction mixture, all the reaction mixtures can be run in one lane of a gel. (Actually only one reaction mix containing all the ddXTP's together need be performed.) The gel can then be scanned by a laser, which detects fluorescence from the dyes, each at a different wavelength.
- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_(OpenStax)/28%3A_Biomolecules_-_Nucleic_Acids/28.06%3A_DNA_SequencingThis product mixture is then separated according to the size of the pieces by gel electrophoresis (Section 26.2), and the identity of the terminal dideoxyribonucleotide in each piece—and thus the sequ...This product mixture is then separated according to the size of the pieces by gel electrophoresis (Section 26.2), and the identity of the terminal dideoxyribonucleotide in each piece—and thus the sequence of the restriction fragment—is determined by noting the color with which the attached dye fluoresces.
