# Lab 8: Sucrose Inversion

Student:

### Writing:

Abstract Introduction

- Theory
- Citation
- Instrumentation

Conclusion

### Data and Results:

Analysis of Raw Data

• Convert rotational angle measurement to concentrations with standard deviations • Linest fit ln[concentration] versus time to obtain a rate constant and error in rate constants • Calculate ln[Concentration] from fit and plot the residual • Use ln[conc+error] and ln[conc-error] for y error bars Further Calculation • From trial 3, 4 and, 5, in part one, create a plot of ln[Rate constant] versus inverse temperature with x and y error bars • Use a linear fit to determine the Activation energy with error bars • Use the seven runs, from part two, to create a plot of inverse rate constant versus inverse sucrose concentration with x and y error bars • Use a linear fit to determine Km and Vmax from slope and y-intercept Discussion: • Did the increase in enzyme have the effect you expected (what did you expect and why)? • Did the change in temp have the effect you expected (what did you expect and why)? • Did the residual plot of rate fit within experimental error? What would improve accuracy of fit? • How does the activation energy compare with thermal energy? Do you expect a spontaneous reaction? • What would improve activation energy determination? • How does run 1 of part 2 compare with the equivalent run of part 1? Comment on reproducibility. • How did you expect substrate concentration to effect your rate (and why), is this what you observed? • What does Km tell you about the reaction? • What is the limiting step of the reaction? What does Vmax tell you about the limiting step? • What part of the calculation affected the error the most? • What measurement (or step) introduces the most error to your final results? Conclusion One paragraph summary