9: Chromatography
- Page ID
- 401486
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- 9.1: Overview of Analytical Separations
- In Chapter 7 we examined several methods for separating an analyte from potential interferents. For example, in a liquid–liquid extraction the analyte and interferent initially are present in a single liquid phase. We add a second, immiscible liquid phase and thoroughly mix them by shaking. During this process the analyte and interferents partition between the two phases to different extents, effecting their separation.
- 9.2: General Theory of Column Chromatography
- Of the two methods for bringing the stationary phase and the mobile phases into contact, the most important is column chromatography. In this section we develop a general theory that we may apply to any form of column chromatography.
- 9.3: Optimizing Chromatographic Separations
- Now that we have defined the solute retention factor, selectivity, and column efficiency we are able to consider how they affect the resolution of two closely eluting peaks.
- 9.4: High-Performance Liquid Chromatography
- In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase.