6.2 Day 6 and 7 Procedures
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Day 6 and 7 - Kinetics Measurements @ 40C and 70C
**CAUTION** Please ensure that you use the cuvettes with Teflon stoppers for your runs ONLY. Cuvettes do not need to be filled more than ~75% full for accurate readings.
Each partner will carry out a kinetic study at another temperature, one higher (e.g., 65 °C) and one lower (e.g., 40 °C). It is desirable to have the minimum and maximum temperatures differ by at least 25 °C, but be aware that a temperature that is too high can lead to a reaction that is inconveniently fast to measure, while a temperature that is too low can lead to a reaction that is inconveniently slow to measure during a given lab period. For these runs monitor the reaction progress with the spectrophotometer set to 550 nm. Select the kinetics icon (not scanning) on the Cary 100 Spectrophotometer and follow the instructions outlined in Appendix 1, part C.
IMPORTANT NOTE: The group will have to come to a consensus on the two temperatures to be studied. One UV-VIS spectrometer will be set to the lower temperature and the other UV-VIS will be set to the higher temperature. Again cooperation will be required, as all teams will need to start their kinetic runs simultaneously. Manually record the time and absorbance at 550 nm for each data point or transfer your data files to a USB drive for analysis.