Skip to main content
Chemistry LibreTexts

Liquid Chromatography/ Capillary Electrophoresis

Definitions: http://www.waters.com/waters/nav.htm?cid=10049080&locale=en_US

Video of instrument in action: http://www.youtube.com/watch? v=kz_egMtdnL4&feature=channel_page

  1. Understand what is going on during partition chromatography. Know how to perform gradient and isocratic elutions.

    http://www.chemistry.nmsu.edu/Instrumentation/Lqd_Chroma.html

    Harvey 12.5.2

  2. Know the differences between normal and reversed phase partition chromatography.

    http://www.chemistry.nmsu.edu/Instrumentation/Lqd_Chroma.html

    Harvey 12.5.1

  3. Know the basic components of an HPLC instrument. Specifically, know the difference between bulk property detectors (refractive index detector) and solute –property detectors (UV-Vis detectors). Also be familiar with the mass spectral detector.

    http://www.instrumentalchemistry.com/liquidphase/pages/sampleloop.htm

    http://www.instrumentalchemistry.com/liquidphase/pages/dampner.htm

    http://www.instrumentalchemistry.com/liquidphase/pages/diodearray.htm

    http://www.instrumentalchemistry.com/liquidphase/pages/fluoresence.htm

    http://www.instrumentalchemistry.com/liquidphase/pages/singlewavelength.htm

    Harvey 12.5.4