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- https://chem.libretexts.org/Courses/California_Polytechnic_State_University_San_Luis_Obispo/Survey_of_Biochemistry_and_Biotechnology/09%3A_Basic_Techniques/9.02%3A_Fractionation_and_Chromatography_TechniquesFractionation of samples, as the name suggests, is a process of separating out the components or fractions of the lysate. Fractionation typically begins with centrifugation of the lysate. Using low-sp...Fractionation of samples, as the name suggests, is a process of separating out the components or fractions of the lysate. Fractionation typically begins with centrifugation of the lysate. Using low-speed centrifugation, one can remove cell debris, leaving a supernatant containing the contents of the cell. By using successively higher centrifugation speeds (and resulting g forces) it is possible to separate out different cellular components, like nuclei, mitochondria, etc., from the cytoplasm.
- https://chem.libretexts.org/Courses/British_Columbia_Institute_of_Technology/Chem_2305%3A_Biochemistry_Instrumental_Analysis/02%3A_Chromatography/2.03%3A_Liquid_Chromatography/2.3.01%3A_High-Performance_Liquid_Chromatography/2.3.1.07%3A_Size-Exclusion_ChromatographySize exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed for...Size exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed forces between the compounds being separated and the surface of the particles used as the stationary phase. Instead, the particles are prepared with well-characterized pore sizes.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Analytical_Sciences_Digital_Library/In_Class_Activities/Separation_Science/5%3A_Liquid_Chromatographic_Separation_Methods/01_Steric_Exclusion_Chromatographyteric exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of steric exclusion separations to be meaningful, there can be no directed ...teric exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of steric exclusion separations to be meaningful, there can be no directed forces between the compounds being separated and the surface of the particles used as the stationary phase. Instead, the particles are prepared with well-characterized pore sizes.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Supplemental_Modules_(Analytical_Chemistry)/Analytical_Sciences_Digital_Library/Courseware/Separation_Science/02_Text/05_Liquid_Chromatographic_Separation_Methods/01_Steric_Exclusion_ChromatographySteric exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of steric exclusion separations to be meaningful, there can be no directe...Steric exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of steric exclusion separations to be meaningful, there can be no directed forces between the compounds being separated and the surface of the particles used as the stationary phase. Instead, the particles are prepared with well-characterized pore sizes.
- https://chem.libretexts.org/Ancillary_Materials/Worksheets/Worksheets%3A_Analytical_Chemistry_II/Size_Exclusion_Chromatography_(Liu)If a column is packed with these porous beads, shown as below, how would this effect the movement of both molecules through the column? Draw a separation chromatogram if a group of these three molecul...If a column is packed with these porous beads, shown as below, how would this effect the movement of both molecules through the column? Draw a separation chromatogram if a group of these three molecules go through this column. Hint: molecular size is directly related to the formula mass of the molecules. If the pore sizes change, what would happen to the calibration curve? What type of applications of size exclusion chromatography can you think of?
- https://chem.libretexts.org/Under_Construction/Purgatory/Principles_of_Instrumental_Analysis_(Skoog_et_al.)_-_Under_Construction/28%3A_High-Performance_Liquid_Chromatography/28.07%3A_Size-Exclusion_ChromatographySize exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed for...Size exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed forces between the compounds being separated and the surface of the particles used as the stationary phase. Instead, the particles are prepared with well-characterized pore sizes.
- https://chem.libretexts.org/Courses/Providence_College/CHM_331_Advanced_Analytical_Chemistry_1/14%3A_Liquid_Chromatography/14.05%3A_Size-Exclusion_ChromatographySize exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed for...Size exclusion chromatography is a technique that separates compounds solely on the basis of size. In order for the results of size exclusion separations to be meaningful, there can be no directed forces between the compounds being separated and the surface of the particles used as the stationary phase. Instead, the particles are prepared with well-characterized pore sizes.
- https://chem.libretexts.org/Ancillary_Materials/Worksheets/Worksheets%3A_Analytical_Chemistry_II/Size_Exclusion_Chromatography_(Crawford_Kloepper)The goal of this activity is to introduce you to size exclusion chromatography, a technique that separates compounds on the basis of size. The pore is bigger than would actually occur and the molecule...The goal of this activity is to introduce you to size exclusion chromatography, a technique that separates compounds on the basis of size. The pore is bigger than would actually occur and the molecules would never have sizes that big relative to the size of the bead. The size of the pore can be controlled to some degree during the synthesis of the bead. Based on your answers above, now draw a chromatogram showing the elution order of the three molecules and label the peaks.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/07%3A_Obtaining_and_Preparing_Samples_for_Analysis/7.11%3A_Chapter_Summary_and_Key_TermsThis chapter summary focuses on the critical aspects of acquiring a representative sample for analysis. It emphasizes the importance of a well-structured sampling plan, including choices about sample ...This chapter summary focuses on the critical aspects of acquiring a representative sample for analysis. It emphasizes the importance of a well-structured sampling plan, including choices about sample types (e.g., random, systematic), collection methods (e.g., grab, composite), and considerations of the population's nature (homogeneous or heterogeneous).
