In Chapter 26 we covered several important elements of chromatography, including the factors that affect the migration of solutes, the factors that contribute to band broadening, and the factors under...In Chapter 26 we covered several important elements of chromatography, including the factors that affect the migration of solutes, the factors that contribute to band broadening, and the factors under our control that we can use to optimize the separation of a mixture. Here we consider two topics that apply to a gas chromatographic separation, both of which are a function of the properties of gases.
The text covers various liquid chromatography techniques, focusing on liquid-solid adsorption, ion-exchange, and size-exclusion chromatography. Liquid-solid chromatography involves polar stationary ph...The text covers various liquid chromatography techniques, focusing on liquid-solid adsorption, ion-exchange, and size-exclusion chromatography. Liquid-solid chromatography involves polar stationary phases and nonpolar solvents for separating compounds, excelling in analyzing isomers. Ion-exchange chromatography uses resin beads with ionic functional groups to separate ions based on their affinity for the exchange sites, utilizing gradient elutions to affect solute retention.
