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- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/28%3A_High-Performance_Liquid_Chromatography/28.05%3A_Adsorption_ChromatographyIn adsorption chromatography (or liquid-solid chromatography, LSC) the column packing also serves as the stationary phase. For most samples, liquid–solid chromatography does not offer any special adva...In adsorption chromatography (or liquid-solid chromatography, LSC) the column packing also serves as the stationary phase. For most samples, liquid–solid chromatography does not offer any special advantages over liquid–liquid chromatography. One exception is the analysis of isomers, where LSC excels.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Instrumental_Analysis_(LibreTexts)/28%3A_High-Performance_Liquid_ChromatographyIn high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column t...In high-performance liquid chromatography (HPLC) we inject the sample, which is in solution form, into a liquid mobile phase. The mobile phase carries the sample through a packed or capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase. By adjusting conditions it usually is possible to design a separation so that analytes elute by themselves, even when the mixture is complex.
- https://chem.libretexts.org/Bookshelves/Analytical_Chemistry/Analytical_Chemistry_2.1_(Harvey)/12%3A_Chromatographic_and_Electrophoretic_Methods/12.01%3A_Overview_of_Analytical_SeparationsThe text discusses methods for separating analytes from interferents, focusing on liquid-liquid extractions and chromatography. Liquid-liquid extractions involve partitioning solutes between two immis...The text discusses methods for separating analytes from interferents, focusing on liquid-liquid extractions and chromatography. Liquid-liquid extractions involve partitioning solutes between two immiscible phases, but face limitations, such as the need for multiple extractions and difficulty separating species with similar distribution ratios. A countercurrent extraction can improve separation efficiency.
- https://chem.libretexts.org/Courses/Los_Angeles_Trade_Technical_College/Analytical_Chemistry/2%3A_Analytical_Chemistry_2.0_(Harvey)/13%3A_Chromatographic/13.1%3A_Overview_of_Analytical_SeparationsDespite the power of liquid–liquid extractions, there are significant limitations. If we have several analytes, we may need to complete a separate extraction for each analyte. A more significant limit...Despite the power of liquid–liquid extractions, there are significant limitations. If we have several analytes, we may need to complete a separate extraction for each analyte. A more significant limitation is that the extent of a separation depends on the distribution ratio of each species in the sample. Other important separations include chromatographic separations and electrophoretic separations.
