The text covers various liquid chromatography techniques, focusing on liquid-solid adsorption, ion-exchange, and size-exclusion chromatography. Liquid-solid chromatography involves polar stationary ph...The text covers various liquid chromatography techniques, focusing on liquid-solid adsorption, ion-exchange, and size-exclusion chromatography. Liquid-solid chromatography involves polar stationary phases and nonpolar solvents for separating compounds, excelling in analyzing isomers. Ion-exchange chromatography uses resin beads with ionic functional groups to separate ions based on their affinity for the exchange sites, utilizing gradient elutions to affect solute retention.
The document provides a comprehensive overview of electrophoresis, focusing notably on capillary electrophoresis (CE). Electrophoresis involves the separation of analytes based on their capacity to mo...The document provides a comprehensive overview of electrophoresis, focusing notably on capillary electrophoresis (CE). Electrophoresis involves the separation of analytes based on their capacity to move through a conductive medium under an electric field, with cations moving towards the cathode and anions towards the anode.