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- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_(OpenStax)/28%3A_Biomolecules_-_Nucleic_Acids/28.07%3A_DNA_SynthesisThe first step in DNA synthesis is to attach a protected deoxynucleoside to a silica (SiO 2 ) support by an ester linkage to the 3′ –OH group of the deoxynucleoside. The second step is removal of the ...The first step in DNA synthesis is to attach a protected deoxynucleoside to a silica (SiO 2 ) support by an ester linkage to the 3′ –OH group of the deoxynucleoside. The second step is removal of the DMT protecting group by treatment with dichloroacetic acid in CH 2 Cl 2 . The reaction occurs by an S N 1 mechanism and proceeds rapidly because of the stability of the tertiary, benzylic dimethoxytrityl cation.
- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_III_(Morsch_et_al.)/28%3A_Nucleic_Acids/28.07%3A_DNA_SynthesisThe occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of th...The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. After the oligonucleotide chain of the desired sequence has been made, the final step is the removal of all the protecting groups and the linkage to silica by reaction with aqueous ammonia (NH 3 ).
- https://chem.libretexts.org/Courses/Martin_Luther_College/Organic_Chemistry_-_MLC/05%3A_Biomolecules/5.04%3A_Biomolecules_-_Nucleic_Acids/5.4.07%3A_DNA_SynthesisThe occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of th...The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. After the oligonucleotide chain of the desired sequence has been made, the final step is the removal of all the protecting groups and the linkage to silica by reaction with aqueous ammonia (NH 3 ).
- https://chem.libretexts.org/Workbench/Pick_Your_Poison%3A_Introduction_to_Materials_Toxicology/26%3A_Biomolecules_-_Nucleic_Acids/26.08%3A_DNA_SynthesisDNA synthesis involves creating new DNA strands, primarily through replication or artificial methods. DNA polymerase plays a crucial role by adding nucleotides complementary to a template strand while...DNA synthesis involves creating new DNA strands, primarily through replication or artificial methods. DNA polymerase plays a crucial role by adding nucleotides complementary to a template strand while ensuring fidelity through proofreading. Techniques such as solid-phase synthesis and PCR are key in labs, enabling advances in gene therapy and synthetic biology. These methods facilitate the manipulation and study of genetic material.
- https://chem.libretexts.org/Bookshelves/Organic_Chemistry/Organic_Chemistry_(Morsch_et_al.)/28%3A_Biomolecules_-_Nucleic_Acids/28.07%3A_DNA_SynthesisThe occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of th...The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. After the oligonucleotide chain of the desired sequence has been made, the final step is the removal of all the protecting groups and the linkage to silica by reaction with aqueous ammonia (NH 3 ).
- https://chem.libretexts.org/Courses/Smith_College/Organic_Chemistry_(LibreTexts)/28%3A_Biomolecules_-_Nucleic_Acids/28.07%3A_DNA_SynthesisThe occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of th...The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. After the oligonucleotide chain of the desired sequence has been made, the final step is the removal of all the protecting groups and the linkage to silica by reaction with aqueous ammonia (NH 3 ).
- https://chem.libretexts.org/Courses/can/CHEM_232_-_Organic_Chemistry_II_(Puenzo)/14%3A_Biomolecules_-_Nucleic_Acids/14.08%3A_DNA_SynthesisThe occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of th...The occurrence of side reactions sets practical limits for the length of synthetic oligonucleotides (up to about 200 nucleotide residues) because the number of errors accumulates with the length of the oligonucleotide being synthesized. After the oligonucleotide chain of the desired sequence has been made, the final step is the removal of all the protecting groups and the linkage to silica by reaction with aqueous ammonia (NH 3 ).
- https://chem.libretexts.org/Workbench/LCDS_Organic_Chemistry_OER_Textbook_-_Todd_Trout/28%3A_Biomolecules_-_Nucleic_Acids/28.08%3A_DNA_SynthesisThe first step in DNA synthesis is to attach a protected deoxynucleoside to a silica (SiO 2 ) support by an ester linkage to the 3′ –OH group of the deoxynucleoside. The second step is removal of the ...The first step in DNA synthesis is to attach a protected deoxynucleoside to a silica (SiO 2 ) support by an ester linkage to the 3′ –OH group of the deoxynucleoside. The second step is removal of the DMT protecting group by treatment with dichloroacetic acid in CH 2 Cl 2 . The reaction occurs by an S N 1 mechanism and proceeds rapidly because of the stability of the tertiary, benzylic dimethoxytrityl cation.
