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All solution preparations are carried out under a fume hood. In this laboratory, the use of acetonitrile and hydrochloric acid required precaution. Acetonitrile is toxic or fatal by inhalation, ingestion or skin absorption. Upon mixing concentrated acid with water heat evolves and precaution was taken in this mixing step. All waste was disposed of in the proper waste container with labels. Protective garment and gloves should be worn at all times.