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Chemistry LibreTexts

Mass Spectrometry Imaging

  • Page ID
    290691
  • Mass spec imaging – lesson plan summary

    • Finish MALDI worksheet from last class (10 min)
    • Short lecture on:
      • MALDI (summarize method, with schematic)
      • SIMS
      • DESI
    • Compare the three methods – ACTIVE PART
    • Applications to mass spec imaging – ACTIVE PART
      • Brief look at leaf and petal paper together on projector

    Comparing MS imaging methods:

    We built a chart on the board of the three methods:

    • Give students the rows, and let them try to fill in the details

     

    Ambient

    Vacuum

    Vacuum

     

    DESI

    MALDI

    SIMS

    Spatial Res

    150 um

    10 um

    0.1 um

    Soft or hard

    Soft

    Soft

    Hard

    Sensitivity (high/low)

    Lower?

    Lower?

    Higher?

    Other

    Non-destructive (can reuse sample)

    Good for proteins.

    Not good for small molecules.

    Deeper penetration.

    My notes:

     

     

    MSI_AmbientVsVacuumOptions.png

    Dozens of ambient ionization options:

    Options.png

    https://www.wur.nl/upload_mm/6/4/6/2b526c3e-2a83-4b32-ae25-3d082b611966_20171005-Intro_Ambient_MSI_Nielen_LR.pdf

    Next, we briefly looked at this paper to see what they did –

    Students pull it up on their laptops

    ImagingLeavesFlowers.png

    J. Agric. Food Chem.201361(31), pp 7477–7487

    https://pubs.acs.org/doi/full/10.1021/jf4011998

     

    Q: What MS imaging technique did they use?

    Ambient or vacuum? 

    Resolution achieved?

    Why might they have done it this way?

    Final Activity (15 min):

    • Had students pull up a computer and get online
    • Search for "mass spec imaging" for a topic they are interested in
      • e.g. biomedical, drug distribution, brain
      • e.g. art conservation, forensic science
      • e.g. food or agriculture
    • Find a paper
      • Which ionization method do they use, and why?
      • How was the sample prepared (live, fixed, stored)?
    • Last 5 - 10 min, have some students share with the class what they found

    This was fun!  Students were very engaged and excited to find such cool science.

    Contributors and Attributions

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