Theory
- Page ID
- 73059
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- Basic Theory
- This section aims to answer the following questions about the basic theory of affinity chromatography: What is affinity chromatography? What types of biological interactions are used in affinity chromatography? What are the steps involved in affinity purification? How do you use affinity purification when a specific ligand for your target molecule is not available? What are the analysis methods for purified biomolecules?
- Applications
- Applications of affinity chromatography covered in this section are as follows: purifying a protein of interest based on histidine-nickel interaction, purifying a protein of interest based on inherent ligand-binding specificity, affinity-based chiral separations, and study of drug interactions.
- Reading
- Further reading on affinity chromatography, including both subscription-based and open-source material.