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Theory

  • Page ID
    73059
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    • Basic Theory
      This section aims to answer the following questions about the basic theory of affinity chromatography: What is affinity chromatography? What types of biological interactions are used in affinity chromatography? What are the steps involved in affinity purification? How do you use affinity purification when a specific ligand for your target molecule is not available? What are the analysis methods for purified biomolecules?
    • Applications
      Applications of affinity chromatography covered in this section are as follows: purifying a protein of interest based on histidine-nickel interaction, purifying a protein of interest based on inherent ligand-binding specificity, affinity-based chiral separations, and study of drug interactions.
    • Reading
      Further reading on affinity chromatography, including both subscription-based and open-source material.


    This page titled Theory is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Contributor.

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