Technique I: Use of Spectrophotometer
Section 1: Purpose of Technique
This technique describes how to use a laboratory spectrophotometer.
Section 2: Operations of Spectronic 200E
There are several types of spectrophotometers that are used to determine the amount of light absorbed within a given sample solution. The spectrophotometers used in the lab are the Spectronic 20, Spectronic 20+ and Spectronic 200. Before using the Spectronic 200, follow these steps to make sure that no damage or injury will occur due to faulty or careless behavior.
NOTE: Several terms are used for the container used to hold a sample in a spectrometer. This procedure will use the term ‘optical cell’ but other terms like ‘cuvette’ may be used in reference texts and instrument manuals.
Make sure the spectrophotometer is on a stable, flat surface free from vibrations.
Plug in the spectrophotometer and turn it on by flipping the power switch at the back of the machine. The following image will appear on the screen when it is on. (Figure 1)
Figure 1. On screen
Make sure that the sample compartment is empty. Then, press ENTER. The following image will appear on the screen. (Figure 2)
Look at the image below. Once at this point, wait for approximately 1 minute.
The screen will then display the Home Menu. Use the arrows to select the SPEC 200E MODERN INTERFACE option, then press ENTER. (Figure 3)
Figure 3. SPEC 200E MODERN INTERFACE
The screen will then show APPLICATION LIVE DISPLAY. Press the right arrow. (Figure 4)
Figure 4. APPLICATION LIVE DISPLAY
The screen will then show APPLICATION MULTI λ, press the right arrow again. (Figure 5)
Figure 5. APPLICATION MULTI λ
Then the screen will show APPLICATION QUANT, press the right arrow again. Refer to figure 6.
Figure 6. APPLICATION QUANT
Then the screen will show APPLICATION ANALYZER, press the right arrow again. Refer to figure 7.
Figure 7. APPLICATION ANALYZER
Then the screen will show APPLICATION SCAN, press ENTER. Refer to figure 8.