5: Determination of cocaine on bills using Gas Chromatography, Mass Spectrometry (GC-MS)
- Page ID
- 401501
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After completing this module, students will be able to:
- Explain the principles of gas-liquid partitioning.
- Predict relative retention on a GC column based on molecular structure.
- Program a GCMS method and operate the instrument to collect useful data.
- Interpret fragmentation patterns of Electron Ionization Mass Spectra.
- Use the mass spectra data library to identify the components in a mixture.
- Determine the response factor of an analyte.
- Quantitate an analyte using an internal standard.
- 5.2: Introduction to Gas Chromatography Instrumentation
- In gas chromatography (GC) we inject the sample, which may be a gas or a liquid, into an gaseous mobile phase (often called the carrier gas). The mobile phase carries the sample through a packed or a capillary column that separates the sample’s components based on their ability to partition between the mobile phase and the stationary phase.
- 5.4: Mass Spectrometers
- A mass spectrometer has three essential needs: a means for producing ions, in this case (mostly) singly charged atoms; a means for separating these ions in space or in time by their mass-to-charge ratios; and a means for counting the number of ions for each mass-to-charge ratio.
- 5.5: Interpreting Electron Ionization Mass Spectra
- This page looks at how fragmentation patterns are formed when organic molecules are fed into a mass spectrometer, and how you can get information from the mass spectrum.
- 5.6: GC-MS Procedure and instructions for data analysis
- This lab involves the analysis of different unknown mixtures by high resolution capillary gas chromatography (GC) with an ion trap detector (ITD). The ITD is a variation of a quadrupole mass spectrometer and is designed to function specifically as a GC detector. Due to the design variances of the ITD compared to a true quadrupole mass spectrometer, the ITD mass spectrum of an organic compound may not be identical (but should be very similar) to its classical electron impact (EI) mass spectrum.