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Chemistry LibreTexts

Liquid Chromatography/ Capillary Electrophoresis


Video of instrument in action: v=kz_egMtdnL4&feature=channel_page

  1. Understand what is going on during partition chromatography. Know how to perform gradient and isocratic elutions.

    Harvey 12.5.2

  2. Know the differences between normal and reversed phase partition chromatography.

    Harvey 12.5.1

  3. Know the basic components of an HPLC instrument. Specifically, know the difference between bulk property detectors (refractive index detector) and solute –property detectors (UV-Vis detectors). Also be familiar with the mass spectral detector.

    Harvey 12.5.4