Skip to main content
Chemistry LibreTexts

Liquid Chromatography/ Capillary Electrophoresis

  • Page ID
    73744
  • \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\)

    Definitions: http://www.waters.com/waters/nav.htm?cid=10049080&locale=en_US

    Video of instrument in action: http://www.youtube.com/watch? v=kz_egMtdnL4&feature=channel_page

    1. Understand what is going on during partition chromatography. Know how to perform gradient and isocratic elutions.

      www.chemistry.nmsu.edu/Instru...qd_Chroma.html

      Harvey 12.5.2

    2. Know the differences between normal and reversed phase partition chromatography.

      www.chemistry.nmsu.edu/Instru...qd_Chroma.html

      Harvey 12.5.1

    3. Know the basic components of an HPLC instrument. Specifically, know the difference between bulk property detectors (refractive index detector) and solute –property detectors (UV-Vis detectors). Also be familiar with the mass spectral detector.

      http://www.instrumentalchemistry.com/liquidphase/pages/sampleloop.htm

      http://www.instrumentalchemistry.com/liquidphase/pages/dampner.htm

      http://www.instrumentalchemistry.com/liquidphase/pages/diodearray.htm

      http://www.instrumentalchemistry.com/liquidphase/pages/fluoresence.htm

      http://www.instrumentalchemistry.com/liquidphase/pages/singlewavelength.htm

      Harvey 12.5.4


    This page titled Liquid Chromatography/ Capillary Electrophoresis is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Contributor.

    • Was this article helpful?