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Chemistry LibreTexts

Chromatography Unit

At the beginning of this unit, I spend essentially an entire class in a lecture format providing background information on chromatography.  This includes some of the history of chromatography beginning with the initial work of Mikhail Tswett, and the introduction of key concepts within chromatography such as the difference between adsorption and partitioning, the distribution constant, the partition coefficient, the selectivity factor, the concept of capacity and the retention factor, the idea of dividing a column into a set of theoretical plates, and retention time.  With this background, I then give them the first problem set.