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Chromatography set #3 – Fundamental Resolution Equation

  • Page ID
    70876
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    1. Describe ways in which the number of plates on a chromatographic column can be increased.Are there any tradeoffs associated with these changes?
    2. Describe ways in which the separation factor can be increased.Are there limits to the effect that increasing the separation factor has on chromatographic resolution?
    3. Describe ways in which the retention or capacity factor can be increased.Are there any tradeoffs associated with these changes?Are there limits to the effect that increasing the retention factor has on chromatographic resolution?
    4. Consider the following chromatogram.

      chromatogram.PNG

      The early eluting peaks and later eluting peaks exhibit a problem.

      1. Describe the chromatographic nature (there is a particular term that describes each) of the problem.
      2. Propose a way in gas chromatography to eliminate both problems.
      3. Propose a way in liquid chromatography to eliminate both problems.

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