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The Nuclear Overhouser Effect (NOE)
The Nuclear Overhauser Effect (NOE) uses a second radio frequency field, usually relatively stronger than the first one, in order to saturate particular resonances. This phenomenon discovered by Albert Overhaouser in 1953.The NOE process can be summarized as a cross relaxation from one spin state to another spin state. The effect causes the perturbation via dipolar interactions with further nucleus spins and increases the intensity of other spins. The effect is called the steady-state Nuclear Overhauser Effect. Since dipolar coupling interacts throughout the space, it is a very useful technique to study the conformation of molecules.
The significant interaction between the magnetic dipoles of the two spins is required for NOE. Because of the fact that dipolar interactions drop of very fast with distance, 1H, 1H NOE can only occur with a distance of <5-6 Å (500-600 pm).
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