Skip to main content
Chemistry LibreTexts

2.1: Experimental Determination of Kinetics

  • Page ID
    • 2.1.1: Continuous Flow
      Continuous Flow is a type of assay used to easily measure the progress of a reaction at discrete time points and is commonly used for determining initial rates and inhibition values.
    • 2.1.2: Measuring Reaction Rates
      The method for determining a reaction rate is relatively straightforward. Since a reaction rate is based on change over time, it must be determined from tabulated values or found experimentally. With the obtained data, it is possible to calculate the reaction rate either algebraically or graphically. What follows is general guidance and examples of measuring the rates of a reaction.
    • 2.1.3: Rate vs. Concentration Proportionalities
    • 2.1.4: Relaxation Methods
      Many chemical reactions are complete in less than a few seconds, which makes the rate of reaction difficult to determine. In these cases, the relaxation methods can be used to determine the rate of the reaction.
    • 2.1.5: Spectrophotometry
      Spectrophotometry is a method to measure how much a chemical substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength. This measurement can also be used to measure the amount of a known chemical substance.
    • 2.1.6: Stopped Flow
      The stopped-flow technique allows for the evaluation of solution-based kinetics on a milliseconds timescale with a very small volume of reactants used.