According to the law of conservation of energy, the muscle energy expanded to pull the opposite charges apart cannot be destroyed. We say that the 0.898 J is gained by the two charges and stored as potential energy. Potential energy (symbol Ep) is the energy which one or more bodies have because of their position. We can always regain this energy by reversing the process during which it was stored. If the two opposite charges are returned to their original separation of 1 cm, their potential energy will decrease by 0.898 J. The energy released will appear as kinetic energy, as heat, or in some other form, but it cannot be destroyed.
If we had taken two particles both of which had a charge of +1 μC for our example of potential energy, work would have been required to push them together against their repulsive force. Their potential energy would increase as they were brought together from the ends of a football field, and 0.898 J would be required to move them to a distance of 1 cm apart. Because the potential energy of like-charged particles increases as they are brought closer together, while that of opposite-charged particles decreases, it is convenient to assign a value of zero potential energy to two charged particles which are a long distance apart. Bringing a pair of positive charges (or a pair of negative charges) closer together increases Ep to a positive value. Bringing one positive and one negative particle together decreases Ep, giving a negative value.