1.41: Molecular Biology in the Clinical Laboratory

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Complementary DNA (cDNA) is best defined as:
1. two DNA strands, each strand consisting of one type of purine base
2. two DNA strands, each strand consisting of one type of pyrimidine base
3. two DNA strands, covalently linked to each other
4. a strand of DNA in which the order of pyrimidine and purine bases complement the opposite base order on a strand of RNA
5. a strand of DNA in which the order of pyrimidine and purine bases complement the opposite base order on a strand of DNA
The polymerase chain reaction (PCR) is a technique used to:
1. increase the sensitivity of enzyme reactions
2. enhance the sensitivity of DNA detection methods
3. cross-ink bonded stationary phases for HPLC
4. measure polymerized collagen in urine
5. degrade DNA to smaller fragments
Analysis of DNA fragments by complementary DNA hybridization is performed by:
1. Northern blot technique
2. Southern blot technique
3. Western blot technique
4. Sandwich technique
5. ELISA technique

Use the following Key to answer Questions 4-5.

1, 2,and 3 are correct
1 and 3 are correct
2 and 4 are correct
only 4 is correct
all are correct

Genetic defects can be detected in vitro by:
1. analysis of DNA
2. measuring amniotic fluid lecithin and sphingomyelin
3. measurement of specific enzyme levels
4. measurement of amniotic fluid bilirubin
Restriction enzymes are:
1. a group of bacterial enzymes
2. enzymes that inhibit DNA replication
3. enzymes that “cut” (hydrolyze) at specific base sequences
4. a group of enzymes that are involved with keeping specific ions outside of the cell.

Answer

e (p. 938)
b (p. 940-941)
b (p. 940941)
b (p. 939)
b (p. 939)