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10. Capillary Electrophoresis (voltage only, no pressure)

  • Page ID
    62646
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    The remaining sections describe how to perform a single Capillary Electrophoresis (CE) analysis on a single sample. This section is a review of Establishing a Baseline for CE. Because the HV Power Supply will be used during this process, please review the Safety Precautions for using high voltage prior to turning on the power supply.

    The data collection software used in Learning Modules I – IV is IGOR Pro 5.0 with NIDAQ Tools by Wavemetrics, Inc. All software references in this section pertain to IGOR Pro. If you are using different data collection software, please refer to the user’s manuals accompaning that software.

    1. To get a baseline, screw the buffer sample vial on to the platform and close the lid. There is a safety switch on the plexi-glass box that prevents current flow in the system if the lid is not closed. Turn on the power supply. Remember that the pressure should be turned off.
    2. Turn on the voltmeter by pressing button labeled “power”. Set the voltmeter to read current. Next, set the separation voltage by turning the knob on the right front of the power supply.
    3. Hit <Start> on the Igor program and monitor the baseline. Look at the voltmeter on top of the power supply to see if the current reading is steady. It should not deviate more than 0.2 mV (2 μA) for a period of 120 seconds.
    4. Turn off the power supply. Hit <Stop> on the Igor program. The base line information does not need to be saved.

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