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Materials

  • Page ID
    64872
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    The buffer, 3-[cyclohexylamino]-1-propanesulfonic acid (CAPs), and analytes n-decanophenone, mesityl oxide, and tolmetin may be replaced. Ideally, the lab should include one charged analyte, a neutral marker, and a micelle marker to allow the students to use critical thinking and laboratory skills to determine capacity factor. The anion we chose to work with maintains charge at pH 10. We selected CAPs as the running buffer because it buffers at pH 10 and provides a low background current. You may choose other systems, for example ascorbic acid or another charged nonsteroidal anti-inflammatory drug. Before asking students to use different chemicals, we recommend you perform the experiments outlined in Learning Module III with your substitute set. If you replace the sodium dodecyl sulfate with another surfactant, or change the pH of the running buffer, you may need to optimize the separation parameters to obtain an acceptable migration time.


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