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Background

  • Page ID
    63685
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    Fundamentals

    In Learning Module III we discussed the separative transport in MEKC and demonstrated how retention factor is derived. In addition, you performed the separations necessary to experimentally determine the capacity factor of a single NSAID. Retention factor can be quite useful for qualitative analysis of an unknown if the experimental procedures result in reproducible data. Quantitative analysis further requires the peak height, or area, is reproducible. The only way to assess this is to participate in a practical exercise, where the composition and concentration of a sample is unknown to you, but is provided following the submission of your results. Gauging your success using a sample for which you can compare your results with known values is critical to developing separation based assays using MEKC.


    This page titled Background is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by Contributor.

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