3.4. Example Supply List
- Page ID
- 133105
\( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}} } \) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash {#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\)
Group 1: % Alcohol and Diacetyl by GC-MS
| Item | Cat. No. | Supplier | Cost |
|---|---|---|---|
| Inlet liner SPME direct | 2637501 | Sigma | $52.00 |
| SPME manual fiber holder | 57330-U | Sigma | $410.00 |
| CAR-PDMS fiber assembly | 57318 | Sigma | $418.00 |
| Headspace vial kit (10 mL PTFE butyl septa) | 27304-U | Sigma | $88.30 |
| Ethanol (ACS reagent) | 459844-500 mL | Sigma | $49.50 |
| 1-propanol | 402893-500mL | Sigma | $42.80 |
| 2,3, butanedione (ACS reagent) | 11038-5 mL | Sigma | $67.50 |
| 2,3 pentanedione | 241962-25g | Sigma | $24.90 |
| NaCl (reagent grade) | 310166-1KG | Sigma | $32.20 |
| Column: SPB-5 capillary column 60 m x 0.32 mmx 1.0 um film thickness | |||
| Hand crimper for 10 mL vials | |||
Group 2: Ca and Mg by Flame AA
| Item | Cat. No. | Supplier | Cost |
|---|---|---|---|
| Hydrochloric acid | 84415-100mL | Sigma | $44.70 |
| Lanthanum Oxide | 289205-100g | Sigma | $244.50 |
| Magnesium ribbon | 13103-25 g | Sigma | $52.80 |
| Calcium carbonate | 202932-5g | Sigma | $46.70 |
Group 3: Chloride and Oxalate Ion by CE
| Item | Cat. No. | Supplier | Cost |
|---|---|---|---|
| Basic anion buffer | 5064-8209 | Agilent | $74.07 |
| NaCl (reagent plus) | S9625-500 g | Sigma | $29.30 |
| Sodium oxalate | 71800-100g | Sigma | $38.30 |
| 50 um id straight capillary | G1600-64211 | Agilent | $94.57 |
| (id = 50 um, l = 104 cm, L = 112.5 cm) | |||
Group 4: Polyphenols (Spectrophotometic and HPLC)
Method 1: Spectrophotometric
| Item | Cat. No. | Supplier | Cost |
|---|---|---|---|
| Ammonium ferric citrate (green) | 09713-50g | Sigma | $29.60 |
| Concentrated ammonia | 320145-500mL | Sigma | $38.60 |
| Carboxymethylcellulose (CMC/EDTA)-low viscosity |
C5678-500g | Sigma | $68.30 |
Method 2: HPLC
| Item | Cat. No. | Supplier | Cost |
|---|---|---|---|
| Hydrochloric acid (37%) | |||
| NaCl (ACS reagent) | S9888-25 g | Sigma | $28.00 |
| ethyl acetate | 676810-1L | Sigma | $80.60 |
| Methanol (HPLC grade) | 439193-4L | Sigma | $108 |
| Water (HPLC grade) | 95304-2.5L | Sigma | $62.20 |
| Formic acid (ACS reagent) | 399288-100mL | Sigma | $36.00 |
| Discovery DPA-6s SPE Tube (1g) | 52629-U | Sigma | $100.50 |
| ferulic acid | 128708-5G | Sigma | $35.50 |
| caffeic acid | C0625-5g | Sigma | $57.10 |
| p-coumaric acid | C9008-5g | Sigma | $21.80 |
Supplies in lab room:
50 mL centrifuge tubes, Shaker, Centrifuge, C18 column (Hypersil BDS C18 column) (5 um particle size, 250 x 4.6 mm i.d.)
Group 5: Bitterness Units (Spectrophotometric and HPLC)
| Item | Cat. No. | Supplier | Cost |
|---|---|---|---|
| International calibration standards | ICE-3 | American Society of Brewing Chemists | $160 |
| DSC-8 SPE tube | 52714-U | Sigma | $93.80 |
| Formic acid (ACS reagent) | 399288-100mL | Sigma | $36.00 |
| Methanol (HPLC grade) | 439193-4L | Sigma | $108 |
| Water (HPLC grade) | 95304-2.5L | Sigma | $62.20 |
| Phosphoric acid (85% wt.) | W290017-sample-K | Sigma | $40.00 |
| C18 column (Hypersil BDS C18 column) (5 um particle size, 250 x 4.6 mm i.d.) | |||