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Section 4: MS-MS and De Novo Sequencing

  • Page ID
    79453
  • Learning Objectives

    At the end of this assignment, you should be able to:

    1. Explain the purpose and methodology of a tandem MS experiment.
    2. Identify b and y ions and use them to determine the sequence of a short peptide by de novo sequencing

    References and Resources

    R. Aebersold and M. Mann, “Mass spectrometry-based proteomics,” Nature, 2003, 422, 198-207.

    The Broad Institute, “MRM (Multiple Reaction Monitoring),” www.broadinstitute.org/scien...ion-monitoring

    F. Klink, “MS Solutions #9: Peptide Sequencing with Electrospray LC/MS Part 1: Ion Types and Nomenclature,” Separation Science, http://www.sepscience.com/Information/Archive/MS-Solutions/244-/MS-Solutions-9-Peptide-Sequencing-with-Electrospray-LCMS-Part-1-Ion-Types-and-Nomenclature

    F. Klink, “MS Solutions #10: Peptide Sequencing with Electrospray LC/MS Part 2: Interpretation of a Simple Spectrum,” Separation Science, http://www.sepscience.com/Information/Archive/All-Articles/246-/MS-Solutions-10-Peptide-Sequencing-with-Electrospray-LCMS-Part-2-Interpretation-of-a-Simple-Spectrum

    Ion Source, “De Novo Peptide Sequencing Tutorial,” 2012, http://ionsource.com/tutorial/DeNovo/DeNovoTOC.htm

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